李超,葛洁,鹿秀梅,李发美
LI Chao,GE Jie,LU Xiu-mei,LI Fa-mei(School of Pharmacy

• 1:沈阳药科大学药学院

摘要(Abstract)：

目的采用RP-HPLC法同时测定骨疏丹颗粒剂中新北美圣草苷、柚皮苷、淫羊藿苷、欧前胡素、蛇床子素5种活性成分。方法采用Diamonsil C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-水为流动相,梯度洗脱,流速1.0 mL.min-1,柱温为室温,检测波长285 nm。结果各个成分的峰面积与其质量浓度的线性关系良好(r>0.9990),加样回收率为95.8%~98.6%。结论该方法简单、准确、重现性好,可为全面评价复方骨疏丹颗粒剂的质量提供依据。
Objective To determine the contents of the neoeriocitrin,icariin,osthole,naringin,imperatorin in Gushudan granules(traditional Chinese medicine).Methods Diamondsil TM C18(250 mm×4.6 mm,5 μm)column was used with a mobile phase of acetonitrile-water;the flow rate was 1.0 mL·min-1;the detection wavelength was set at 285 nm.Results The linearity was obtained with good result(r<0.999 0).The average recoveries of five active constitutes in Gushudan granules were all in 95.8%-98.6%,and the RSD were less than 2.1%.Conclusions The validated method has the advantages of simplicity,precision and reliability,allowing quality control of Gushudan granules.

关键词(KeyWords)： 反相高效液相色谱法;新北美圣草苷;柚皮苷;淫羊藿苷;欧前胡素;蛇床子素;骨疏丹
RP-HPLC;neoeriocitrin;icariin;osthole;naringin;imperatorin;Gushudan granule

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(30472168)

作者(Author): 李超,葛洁,鹿秀梅,李发美
LI Chao,GE Jie,LU Xiu-mei,LI Fa-mei(School of Pharmacy

参考文献(References)：

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