冯秀丽,许庆华,赵晓云,刘畅,陈羽,徐威
FENG Xiu-li,XU Qing-hua,ZHAO Xiao-yun,LIU Chang,CHEN Yu,XU Wei(School of Life Science and Bio-pharmaceutics

• 1:沈阳药科大学生命科学与生物制药学院

摘要(Abstract)：

目的观察金银花及其复方仙方活命饮水提液对金黄色葡萄球菌、大肠埃希菌、枯草芽孢杆菌、白色念珠菌和黑曲霉的体外抑菌活性,探讨两者对小鼠抗炎作用及对炎性细胞的影响,并考察两者对小鼠免疫器官的影响。方法采用琼脂扩散法测定金银花及其复方仙方活命饮的水煎提取液对5种菌的抑菌能力。用试管二倍稀释法与平板涂布法结合确定药液对5种菌的最小抑菌浓度(MIC)。以二甲苯诱发的小鼠耳肿胀炎症作为急性炎症动物模型,进行抗炎作用研究。结果金黄色葡萄球菌、大肠埃希菌、枯草芽孢杆菌、白色念珠菌对金银花水提液与仙方活命饮水提液均敏感;黑曲霉对金银花水提液敏感,对仙方活命饮水提液不敏感。金银花水提液与仙方活命饮水提液,均能抑制二甲苯所致小鼠耳肿胀反应,提高小鼠脾脏指数,降低血清中白细胞介素-6与肿瘤坏死因子-α的含量。结论金银花及其复方仙方活命饮有抑菌、抗炎作用,且复方的功效强于单味药。
Objective To study the anti-bacterial effect in vitro and the anti-inflammatory effects in vivo of extract from Flos Lonicerae Japonicae and Fairy Formula Decoction for Saving Life against Staphylococcus aureus,Escherichia coli,Bacillus subtilis,Candia albicans and Aspergillus migr.Methods Agar diffusion method and broth double dilution method were used to measure the bactriostasis of extracts from Flos Lonicerae Japonicae and Fairy Formula Decoction for Saving Life.The ear edema induced by xylene was used as an animal model of acute inflammation.Results Staphylococcus aureus,Escherichia coli,Bacillus subtilis and Candia albicans had sensitivity for extracts from Flos Lonicerae Japonicae and Fairy Formula Decoction for Saving Life,Aspergillus miger was sensitive for Flos Lonicerae Japonicae,but not sensitive for Fairy Formula Decoction for Saving Life.Both extracts from Flos Lonicerae Japonicae and Fairy Formula Decoction for Saving Life significantly inhibited the ear edema,increased spleen index number,and decreased levels of IL-6 and TNF-α in serum.Conclusions Both extracts from Flos Lonicerae Japonicae and Fairy Formula Decoction for Saving Life have anti-bacterial and anti-inflammatory effect.Fairy Formula Decoction for Saving Life extract has stronger effect than Flos Lonicerae Japonicae.

关键词(KeyWords)： 金银花;仙方活命饮;抑菌;抗炎
Flos Lonicerae Japonicae;Fairy Formula Decoction for Saving Life;anti-bacterial effect;anti-inflammatory

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 冯秀丽,许庆华,赵晓云,刘畅,陈羽,徐威
FENG Xiu-li,XU Qing-hua,ZHAO Xiao-yun,LIU Chang,CHEN Yu,XU Wei(School of Life Science and Bio-pharmaceutics

DOI: 10.14066/j.cnki.cn21-1349/r.2013.01.002

参考文献(References)：

• [1]李睿明,姜巍.应重视抗菌中药复方的研究[J].医学与哲学,2000,21(10):39-40.
• [2]王盈峰,王登良.金银花中主要内含物最佳浸提条件研究[J].安徽农业科学,2007,35(25):7761-7762.
• [3]李京晶,籍保平,李博,等.十味中草药提取液的体外抗菌活性研究[J].食品科学,2006,27(4):148-150.
• [4]李建志,刘旭红,杨丽珍,等.11种中草药抗菌作用实验研究[J].中医药信息,2009,26(3):82-83.
• [5]刘春云,武廷章,周大喜,等.凤丹丹皮酚抗菌作用的研究[J].生物学杂志,2000,17(3):23-24.
• [6]马建凤,刘华钢,朱丹.中药体外抑菌研究方法学进展[J].药物评价研究,2010,33(1):42-45.
• [7]DENG Jia-gang,WANG Shuo,GUO Li-cheng,et al.Anti-inflammatory and analgesic effects of extract from rootsand leaves of Citrullus lanatus[J].Chinese Herbal Medicines,2010(3):231-235.
• [8]杨凤蕊,张金梁,崔志清.大承气颗粒抗小鼠急性细菌性腹膜炎作用的研究[J].中药药理与临床,2009,25(3):3-6.
• [9]祁林,刘丽芳.黄丹凝胶抗炎及免疫抑制作用的实验研究[J].中华中医药学刊,2008,26(5):1007-1009.
• [10]陈虹,刘磊,吴润,等.12种中草药对8种畜禽肠道病原菌的体外抑菌试验[J].甘肃农业大学学报,2009,44(3):26-30.
• [11]范文昌,梅全喜,欧秀华,等.12种广东地产清热解毒药材的抗炎作用研究[J].中国药业,2011,20(8):28-30.
• [12]卞如濂.抗炎免疫药理学与临床应用[M].北京:医科大学中国协和大学联合出版社,1992:127.
• [13]张士侠,李心,尹家乐,等.江苏地产自首乌C21甾苷抗炎作用及其机制研究[J].中国现代中药,2007,9(5):8-12.