伍庆,乙引,杨建华,邹晶
WU Qing1,YI Yin1,YANG Jian-hua2,ZOU Jing1(1.Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment

• 1:贵州省山地环境信息系统与生态环境保护重点实验室
• 2:贵州华泰药业有限公司

摘要(Abstract)：

目的建立同时测定痔痛安搽剂中三种蒽醌类成分大黄素、大黄酚和大黄素甲醚含量的方法,提高制剂的质量控制标准。方法采用HPLC法。色谱柱为Diamonsil C18柱,流动相为甲醇-质量分数为0.1%的磷酸水溶液(体积比75∶25),流速1.0 mL.min-1,检测波长254 nm,柱温35℃。结果大黄素、大黄酚、大黄素甲醚质量浓度分别在0.64~20.48 mg.L-1(r=0.9995)、0.31~9.84 mg.L-1(r=0.9997)、0.50~16.08 mg.L-1(r=0.999 8)内与峰面积呈良好的线性关系;大黄素平均加样回收率为98.8%,大黄酚平均加样回收率为99.6%,大黄素甲醚平均加样回收率为98.5%。结论采用HPLC法可同时测定痔痛安搽剂中三种蒽醌类成分大黄素、大黄酚和大黄素甲醚的含量,且操作方法简便,分离效果好,运行成本低。该法可用于痔痛安搽剂的质量评价。
Objective To develop a method for determination of emodin,chrysophanol and physcion in Zhitong'an(traditional Chinese medicines) liniment.Methods RP-HPLC method was used with Diamonsil C18(250 mm×4.6 mm,5 μm) column.The mobile phase was methanol-0.1% phosphoric acid(V∶V=75∶25),flow rate was 1.0 mL·min-1.The detection wavelength was set at 254 nm,and column temperature was 35 ℃.Results The calibration curves were linearin the range of 0.6420.48 mg·L-1(r=0.999 5)for emodin,0.31-9.84 mg·L-1(r=0.999 7) for chrysophanol and 0.50-16.08 mg·L-1(r=0.999 8) for physcion.The average recoveries of emodin,physcion and chrysophanol were 98.8%,99.6% and 98.5%,respectively.Conclusions The method is convenient and appropriate for determination of emodin,chrysophanol and physcion in Zhitong'an liniment.

关键词(KeyWords)： 痔痛安搽剂;大黄素;大黄酚;大黄甲醚;含量测定;高效液相色谱法
Zhitong'an liniment;emodin;chrysophanol;physcion;content determination;HPLC

Abstract:

Keywords:

基金项目(Foundation): 贵州省中药现代化专项基金资助项目(20055034)

作者(Author): 伍庆,乙引,杨建华,邹晶
WU Qing1,YI Yin1,YANG Jian-hua2,ZOU Jing1(1.Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment

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