王玉波;李丽燕;
Wang Yubou Li Liyan Department of Antibiotics

• 1:沈阳药学院微生物制药
• 2:沈阳药学院抗生素教研室 46期学生

摘要(Abstract)：

本实验用分光光度法对麦迪霉素的稳定性进行研究。结果表明在55℃、70℃,于pH2～9范围内稳定,在50℃、55℃,65℃,pH=1.0,pH=12.0不稳定。其水解反应服从一级反应动力学公式,测得:pH=12.0:50℃,K=4.00×10^(-3)min(-3)min^(-1),55℃,K=7.68×10(-1),55℃,K=7.68×10^(-3)min(-3)min^(-1),65℃,K=4.12×10(-1),65℃,K=4.12×10^(-2)min(-2)min^(-1);pH=1.0:50℃,K=1.76×10(-1);pH=1.0:50℃,K=1.76×10^(-3)min(-3)min^(-1),55℃,K=6.41×10(-1),55℃,K=6.41×10^(-3)min(-3)min^(-1),65℃,K=2.14×10(-1),65℃,K=2.14×10^(-2)min(-1)。并证明温度对反应速度的影响遵守阿累尼乌斯公式。求出pH=12.0,E_a=3.27×10(-2)min(-1)。并证明温度对反应速度的影响遵守阿累尼乌斯公式。求出pH=12.0,E_a=3.27×10⁴cal/mol,A=3.98×104cal/mol,A=3.98×10⁽¹⁹⁾;pH=1.0,E_a=3.43×10(19);pH=1.0,E_a=3.43×10⁴cal/mol,A=3.98×104cal/mol,A=3.98×10⁽²⁰⁾,并建立具体的阿累尼乌斯公式: pH=12.0:1gK=-7.1×10(20),并建立具体的阿累尼乌斯公式: pH=12.0:1gK=-7.1×10³·_T3·_T¹+19.6 pH=1.0:1gK=-7.5×101+19.6 pH=1.0:1gK=-7.5×10³·(1/T)+20.6
In the experiment, we studied the stability of medemycin by means of spectrophotometry.The results showed that medemycin is stable at 55℃, 70℃ over a pH range of 2—9, but not stable at 50℃,55℃, 65℃, or when pH=1.0,pH=12. The hydrolytic reaction of medemycin complies with a first—order reaction of kinetic equation.According to the kinetic equation, we determined: pH=12 50℃, K=4.00×103·(1/T)+20.6
In the experiment, we studied the stability of medemycin by means of spectrophotometry.The results showed that medemycin is stable at 55℃, 70℃ over a pH range of 2—9, but not stable at 50℃,55℃, 65℃, or when pH=1.0,pH=12. The hydrolytic reaction of medemycin complies with a first—order reaction of kinetic equation.According to the kinetic equation, we determined: pH=12 50℃, K=4.00×10^(-3)min(-3)min^(-1), 55℃, K=7.68×10(-1), 55℃, K=7.68×10^(-3)min(-3)min^(-1) 65℃, K=4.12×10(-1) 65℃, K=4.12×10^(-2)min(-2)min^(-1) pH=1.0 50℃, K=1.76×10(-1) pH=1.0 50℃, K=1.76×10^(-3)min(-3)min^(-1), 55℃, K=6.41×10(-1), 55℃, K=6.41×10^(-3)min(-3)min^(-1), 65℃, K=2.14×10(-1), 65℃, K=2.14×10^(-2)min(-2)min^(-1), It was also proved that the action of temperature on the rate constant complies Arrhenius equation.Calculating E. and A for the Arrhenius equation: pH=12, E_a=3.27×10(-1), It was also proved that the action of temperature on the rate constant complies Arrhenius equation.Calculating E. and A for the Arrhenius equation: pH=12, E_a=3.27×10⁴cal/mol A=3.98×104cal/mol A=3.98×10⁽¹⁹⁾ pH=1.0 E_s=3.43×10(19) pH=1.0 E_s=3.43×10⁴cal/mol A=3.98×104cal/mol A=3.98×10⁽²⁰⁾ Then the Arrhenius equation was definitely established: pH=12 lg K=-7.1×10(20) Then the Arrhenius equation was definitely established: pH=12 lg K=-7.1×10³1/T+19.6 pH=1.0 lg K=-7.5×1031/T+19.6 pH=1.0 lg K=-7.5×10³1/T+20.6

关键词(KeyWords)： 麦迪霉素;稳定性;一级反应;动力学方程;速度常数
Medemycin; First-order reaction; Stability; kinetic equation; Rate constant

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 王玉波;李丽燕;
Wang Yubou Li Liyan Department of Antibiotics

参考文献(References)：

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• 2． 任钧如:抗生素 6:66,1981
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• 4． 方纲主编:抗菌素生物理化特性,人民卫生出版社,第二分册,173页,1981