红霉素链霉菌遗传控制的研究 一 红霉素链霉菌无活性突变株回复突变株的筛选STUDIES ON GENETIC MANIPULATION OF STREPTOMYCES ERYTHREUS 1. Selection of the Revertant of A Zero producer of Streptomyces Erythreus
熊宗贵;陈桂琴;郭锐;陆泰华;
Xiong Zongqui Chen Guiqin Guo Rui Lu Taihua Department of Antibiotics
摘要(Abstract)：
利用亚硝基胍(NTG)对红霉素链霉菌(S.erythreus UV 80)活性菌株进行回复突变,获得了红霉素高产量变株。先以NTG(100μg/ml,1小时)处理母株,得无活性变株,再经诱变,得到回复突变株,其活性提高8％。进一步用NTG(1000μg/ml,1小时)诱变处理,得到了比原菌株红霉素产量高25％的变株。诱变剂的最适剂量为产生90～94％死亡率的剂量。同时还观察到红霉素链霉菌变株的培养特征和生产能力之间有相关性。
The higher yielding strain was obtained through the reverse mutant of a zero producer of Streptomyces erythreus UV 80 by NTG treatment.To start with NTG treatment(1000μg/ml,lhr)zero mutants were prodruced and followed by NTG treatment again,a revertant showing a 8% higher activity was obtained.The revertant treated further by NTG(1000μg/ml,lhr),the resulted mutant yielded 25% more erthromycin than the original strain.The optimal dose of NTG mutagen was that of producing 90—94% death-rate.At the same time the correlation between the cultural characteristics and productivity of S. erythreus mutants was observed.
关键词(KeyWords)：
红霉素链丝菌;诱变;红霉素
Streptomyces erythreus; Mutation;Erythromycin
基金项目(Foundation):
作者(Authors):
熊宗贵;陈桂琴;郭锐;陆泰华;
Xiong Zongqui Chen Guiqin Guo Rui Lu Taihua Department of Antibiotics
参考文献(References)：
- 1． Martin JF and Demain AL: Microbiol Rev 44(2) :230, 1980
- 2． 熊宗贵等:抗生素 7(2) :111,1982
- 3． Spirek J, et al: Antimicrob Agents Chemother 138,1965
- 4． Corcoran JW: S-Adenosylmethionine: Erythromycin C o-methyltransferase In: Hash JH, ed Methods in enzymology Vol XLⅢ, Antibiotics pp487-497,1975
- 5． Delic V, et al: J Gen Microbiol 55:103,1969
- 6． 汤建中等:金霉素链霉菌无活性菌株回复突变的研究,微生物学通报7(5) :205,1980
- 7． Unowsky J and Hoppe DC:J Antibiot 31:662,1978
- 8． Dulaney EL and Dulaney DD: Trans N Y Acad Sci 29(11) :782,1967
- 9． Trilli A, et al: J Chem Tech Biotechnol 32(1) :281,1982