刘学贵,张文超,金梅,吴志宇,牟晓锟,高品一
LIU Xue-gui,ZHANG Wen-chao,JIN Mei,WU Zhi-yu,MU Xiao-kun,GAO Pin-yi

• 1:沈阳化工大学制药与生物工程学院
• 2:沈阳化工大学化学工程学院

摘要(Abstract)：

目的对金樱子中的三萜类化学成分进行研究。方法采用制备高效液相色谱、重结晶等分离纯化方法对金樱子果实的乙醇提取物进行成分分离纯化,利用1H-NMR、13C-NMR、ESI-MS波谱法对分离得到的化合物进行结构鉴定。结果从金樱子中得到12个三萜化合物,分别鉴定为1α,2α,3β,19α-四羟基-12-烯-28-乌苏酸(1α,2α,3β,19α-tetrahydroxyurs-12-en-28-oic acid,1)、2α,3α,19α,23-四羟基-12-烯-28-乌苏酸(2α,3α,19α,23-tetrahydroxyurs-12-en-28-oic acid,2)、2α,3α,19α-三羟基-12-烯-28-乌苏酸(2α,3α,19α-trihydroxyurs-12-en-28-oic acid,3)、2α,3β,19α,23-四羟基-12-烯-28-乌苏酸(2α,3β,19α,23-tetrahydroxyurs-12-en-28-oic acid,4)、19α-羟基亚细亚酸-28-O-β-D-吡喃葡萄糖苷(19α-hydroxyasiatic acid-28-O-β-D-glucopyrannoside,5)、白桦酯酸(betulinic acid,6)、2α,3β-二羟基羽扇-20-烯-28-酸(2α,3β-dihydroxylup-20-en-28-oic acid,7)、2α,3β-二羟基羽扇-20-烯-28-酸甲酯(2α,3β-dihydroxylup-20-en-28-acid methyl ester,8)、3-O-反-对-香豆酰基麦珠子酸(3-O-trans-p-coumaroyl alphitolic acid,9)、3-O-顺-对-香豆酰基麦珠子酸(3-O-cis-p-coumaroyl alphitolic acid,10)、3-O-反-对-香豆酰基马斯里酸(3-O-trans-p-coumaroyl maslinic acid,11)和3-O-顺-对-香豆酰基马斯里酸(3-O-cis-p-coumaroyl maslinic acid,12)。结论化合物8和9为首次从蔷薇科中分离得到,化合物10~12为首次从植物金樱子中分离得到。
Objective To investigate the chemical constituents of Rosa laevigata Michx. Methods Various techniques including column chromatography over silica gel,and HPLC w ere employed for isolation and purification of ethanol extract of R. laevigata Michx. The structures w ere identified by spectroscopic analyses including1H-NMR,13C-NMR and ESI-MS. Results Tw elve triterpenoids w ere isolated and their structure w ere identified as 1α,2α,3β,19α-tetrahydroxyurs-12-en-28-oic acid (1),2α,3α,19α,23-tetrahydroxyurs-12-en-28-oic acid (2),2α,3α,19α-trihydroxyurs-12-en-28-oic acid (3),2α,3β,19α,23-tetrahydroxyurs-12-en-28-oic acid (4),19α-hydroxyasiatic acid-28-O-β-D-glucopyrannoside (5),betulinic acid (6),2α,3β-dihydroxylup-20-en-28-oic acid (7),2α,3β-dihydroxylup-20-en-28-acid methyl ester (8),3-O-trans-p-coumaroyl alphitolic acid (9),3-O-cis-p-coumaroyl alphitolic acid (10),3-O-trans-p-coumaroyl maslinic acid( 11),3-O-cis-p-coumaroyl maslinic acid (12). Conclusions Compounds 8 and 9 are isolated from the Rosaceae for the first time,compounds 10-12 are isolated from the plant for the first time.

关键词(KeyWords)： 金樱子;三萜;分离;化学成分;结构鉴定
Rosa laevigata Michx.;triterpenoid;isolation;chemical constituent;structure identification

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 刘学贵,张文超,金梅,吴志宇,牟晓锟,高品一
LIU Xue-gui,ZHANG Wen-chao,JIN Mei,WU Zhi-yu,MU Xiao-kun,GAO Pin-yi

DOI: 10.14066/j.cnki.cn21-1349/r.2013.11.005

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