HPLC法同时测定蛇床子中蛇床子素和欧前胡素含量Determination of the osthole and imperatorin in Fructus Cnidii by RP-HPLC
孟繁浩,李遇伯,杨正毅,张丽娜,李发美
MENG Fan-hao; LI Yu-bo; YANG Zheng-yi; ZHANG Li-na; LI Fa-mei (School of Pharmacy;
摘要(Abstract)：
目的用高效液相色谱法同时测定中药蛇床子中蛇床子素和欧前胡素的含量。方法采用KromasilC8(2 5 0mm× 4 6mm ,5 μm)为色谱柱 ,以甲醇 水 (体积比 6 5∶35 )为流动相的HPLC法 ,检测波长为 310nm ,流速为 0 8mL·min-1,柱温为室温。结果以醋酸氟轻松为内标 ,蛇床子素和欧前胡素分别在 13～ 2 0 8mg·L-1和 8～ 12 8mg·L-1内 ,线性关系良好 (r≥ 0 9998) ;平均回收率分别为 98 2 %和 97 5 % ;RSD分别为 1 7%和 2 0 % (n =9)。结论本法简便快速 ,回收率及重现性良好 ,适用于中药蛇床子中蛇床子素和欧前胡素的定量分析
Objective To develop a method for determination of osthole and imperatorin in Fructus Cnidii by RP-HPLC.Methods Chromatography was performed using a Kromasil C 8( 250 mm× 4.6 mm, 5 μm) column with mobile phase of methanol-water (65∶35).The monitoring wavelength was 310 nm.The flow rate was 0.8 mL·min -1 and the column temperature was room temperature.fluocinonide was served as an internal standard.Results A good linearity between peak area ratio of the common peak to the internal standard and the concentration was found in the range of 13～ 208 mg·L -1 for osthole and 8～ 128 mg·L -1 for osthole ( r>0.999 8) and imperatorin ( r>0.999 9),respectively.The average recoveries were 98.2% (n=9,RSD=1.7%) and 97.5%(n=9,RSD=2.0%) for osthole and imperatorin,respectively.Conclusion The method is simple,rapid and with satisfactory recoveries and good reproducibilities.It can be used to control the quality of Fructus Cnidii.
关键词(KeyWords)：
蛇床子;蛇床子素;欧前胡素;高效液相色谱法
Fructus Cnidii; osthole; imperatorin; HPLC
基金项目(Foundation):
作者(Authors):
孟繁浩,李遇伯,杨正毅,张丽娜,李发美
MENG Fan-hao; LI Yu-bo; YANG Zheng-yi; ZHANG Li-na; LI Fa-mei (School of Pharmacy;
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