董自艳,彭缨,黄怡,李迅,赵玲誉,张玮
DONG Zi-yan1,PENG Ying1,HUANG Yi1,LI Xun2,ZHAO Ling-yu1,ZHANG Wei1 (1.School of Pharmacy

• 1:沈阳药科大学药学院
• 2:格林制药有限公司

摘要(Abstract)：

目的建立HPLC法同时测定朝鲜淫羊藿提取物中淫羊藿苷A、淫羊霍苷、朝藿定B、淫羊藿次苷Ⅱ含量的方法。方法色谱柱为Diamonsil C18(250 mm×4.6 mm,5μm),流动相为甲醇-水梯度洗脱[0~25 min,w(甲醇)=55%~70%;25~40 min,w(甲醇)=70%~90%;40~45 min,w(甲醇)=90%~55%;45~60 min,w(甲醇)=55%],流速为0.8 mL.min-1,检测波长为270 nm,柱温为25℃。结果淫羊藿苷A、淫羊霍苷、朝藿定B、淫羊藿次苷Ⅱ的线性关系良好,线性范围分别为16.5~82.5、96.0~480.0、30.6~153.0、1.5~7.5 mg.L-1,平均回收率分别为103.7%、100.9%、102.2%、100.1%,RSD分别为1.4%、2.9%、1.2%、2.5%(n=5)。结论该方法可作为朝鲜淫羊藿提取物的含量测定方法 ,为其质量控制提供参考依据。
Objective To develop a method for the quantitative analysis of epimedoside A,icariin,epimedin B and icariside Ⅱ in the extract of Epimedium koreanum Nakai.Methods The extract was determined by RP-HPLC.A Diamonsil-C18 column(250 mm×4.6 mm,5 μm)was used;The mobile phase consisted of methanol and water,gradient elution[0-25 min,w(methanol)=55%-70%;25-40 min,w(methanol)=70%-90%;40-45 min,w(methanol)=90%-55%;45-60 min,w(methanol)=55%];The flow rate was 0.8 mL·min-1.The UV detection was set at 270 nm.The column temperature was 25 ℃.Results The linear response ranges were 16.5-82.5,96.0-480.0,30.6-153.0,1.5-7.5 mg·L-1,respectively.The recovery of the four compounds were 103.7%,100.9%,102.2%,100.1%,respectively,and the relative standard deviations(RSDs)were less than 3.0%(n=5).Conclusions This method can be used for the quality control of the extract of Epimedium koreanum Nakai.

关键词(KeyWords)： 淫羊藿苷A;淫羊霍苷;朝藿定B;淫羊藿次苷Ⅱ;朝鲜淫羊藿;反相高效液相色谱法
epimedoside A;icariin;epimedin B;icariside Ⅱ;Epimedium koreanum Nakai;RP-HPLC

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 董自艳,彭缨,黄怡,李迅,赵玲誉,张玮
DONG Zi-yan1,PENG Ying1,HUANG Yi1,LI Xun2,ZHAO Ling-yu1,ZHANG Wei1 (1.School of Pharmacy

DOI: 10.14066/j.cnki.cn21-1349/r.2010.08.001

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