李艳春;张鹏飞;罗杞瑜;邓意辉;马恩龙;景永奎;
LI Yan-chun1,ZHANG Peng-fei1,LUO Qi-yu1,DENG Yi-hui2,MA En-long1,JING Yong-kui1(1.School of Life Science and Biopharmaceutics,Shenyang Pharmaceutical University,Shenyang 110016,China;2.School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China)

• 1:沈阳药科大学生命科学与生物制药学院
• 2:沈阳药科大学药学院

摘要(Abstract)：

目的研究葫芦素E(cucurbitacin E,CuE)体外对人肝癌BEL7402和HepG2细胞的增殖抑制作用及机制。方法MTT法检测不同浓度CuE体外对BEL7402和HepG2细胞的增殖抑制作用;流式细胞仪检测CuE对细胞周期分布的影响;Western blot法检测CuE对细胞周期调节蛋白cy-clinB1、Cdc25C、Cdk1及p21表达的影响。结果CuE作用72 h可剂量依赖性地抑制BEL7402和HepG2细胞增殖;CuE(100 nmo.lL-1)作用12、24 h后可使BEL7402和HepG2细胞周期阻滞于G2/M期,并可下调Cdk1蛋白的表达,上调p21蛋白的表达。结论CuE体外可抑制人肝癌BEL7402和HepG2细胞的增殖,其增殖抑制作用与诱导G2/M周期阻滞有关。
Objective To investigate the proliferation inhibitory effect and mechanism of cucurbitacin E(CuE)in hepatoma BEL7402 and HepG2 cells.Methods BEL7402 and HepG2 cells were treated respectively with CuE,and then the proliferation inhibitory rate was measured by MTT assay;the change of cel1 cycle distribution was determined by flow cytometry(FCM);the expression of cell cycle-related proteins,such as cyclin B1,Cdc25C,Cdk1 and p21 were detected by Western blot assay.Results CuE inhibited the proliferation of both BEL7402 and HepG2 cells at concentrations of 10-300 nmol·L-1 without causing cytotoxicity;the cell cycle of BEL7402 and HepG2 cells was arrested at G2/M phase after treatment of CuE for 12 and 24 h;Western blot assay showed that Cdk1protein was down-regulated and p21 protein was upregulated after CuE treatment.Conclusions CuE inhibits the proliferation of BEL7402 and HepG2 cells through induction of G2/M phase arrest.

关键词(KeyWords)： 葫芦素E;肝癌细胞;G2/M阻滞
cucurbitacin E;hepatoma cell;G2/M arrest

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 李艳春;张鹏飞;罗杞瑜;邓意辉;马恩龙;景永奎;
LI Yan-chun1,ZHANG Peng-fei1,LUO Qi-yu1,DENG Yi-hui2,MA En-long1,JING Yong-kui1(1.School of Life Science and Biopharmaceutics,Shenyang Pharmaceutical University,Shenyang 110016,China;2.School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China)

DOI: 10.14066/j.cnki.cn21-1349/r.2010.05.011

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