王彧博;郑立;李文;裴月湖;
WANG Yu-bo1,ZHENG Li2,LI Wen3,PEI Yue-hu1(1.School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China;2.Qingdao Key Laboratory of Analytical Technology Development and Standardization of Chinese Medicines,First Institute of Oceanography,State Oceanic Administration,Qingdao 266061,China;3.The Test Center for Analysis,Shenyang Pharmaceutical University,Shenyang 110016,China)

• 1:沈阳药科大学中药学院
• 2:国家海洋局第一海洋研究所青岛市现代分析技术及中药标准化重点实验室
• 3:沈阳药科大学分析测试中心

摘要(Abstract)：

目的研究海洋细菌NJ6-3-1次级代谢产物,以期得到有活性的先导化合物。方法采用硅胶柱色谱、凝胶柱色谱、HPLC等方法进行分离纯化,通过理化性质和波谱手段分析确定化合物的结构。结果从海洋细菌NJ6-3-1的乙酸乙酯萃取物中分离得到15个化合物,分别为环(色-脯)二肽(cyclo(Trp-Pro),1)、环(甘-脯)二肽(cyclo(Gly-Pro),2)、环(甘-苯丙)二肽(cyclo(Gly-Phe),3)、环(丙-苯丙)二肽(cylo(Ala-Phe),4)、环(酪-苯丙)二肽(cyclo(Tyr-Phe),5)、环(酪-脯)二肽(cy-clo(Tyr-Pro),6)、环(4-羟基-脯氨酸-苯丙氨酸)(cyclo(4-hydroxyl-Pro-Phe),7)、环(4-羟基-脯氨酸-亮氨酸)(cyclo(4-hydroxyl-Pro-Leu),8)、环(酪-亮)二肽(cyclo(Tyr-Leu),9)、环(丙-亮)二肽(cyclo(Ala-Leu),10)、环(甘-亮)二肽(cyclo(Gly-Leu),11)、环(丙-缬)二肽(cyclo(Ala-Val),12)、异光黄素(isolumichrome,13)、胸腺嘧啶(thymine,14)、尿嘧啶(uracil,15)。结论化合物1¹5均为首次从海洋细菌NJ6-3-1次级代谢物中分离得到。
Objective To study the chemical constituents from marine bacteria NJ6-3-1.Methods The compounds from the ethyl acetate extracts were isolated by silica gel column,Sephadex LH-20 column and HPLC,and their structures were identified by 1H-NMR,13C-NMR and comparison of chemical and physical data with authentic samples reported in literatures.Results Fifteen compounds were isolated from the ethyl acetate extracts of marine bacteria NJ6-3-1.They were identified as cyclo(Trp-Pro)(1),cyclo(Gly-Pro)(2),cyclo(Gly-Phe)(3),cylo(Ala-Phe)(4),cyclo(Tyr-Phe)(5),cyclo(Tyr-Pro)(6),cyclo(4-hydroxyl-Pro-Phe)(7),cyclo(4-hydroxyl-Pro-Leu)(8),cyclo(Tyr-Leu)(9),cyclo(Ala-Leu)(10),cyclo(Gly-Leu)(11),cyclo(Ala-Val)(12),isolumichrome(13),thymine(14),uracil(15).Conclusions All of the compounds are obtained from marine bacteria NJ6-3-1 for the first time.

关键词(KeyWords)： 海洋细菌;乙酸乙酯萃取物;化学成分;结构鉴定
marine bacteria;ethyl acetate extract;chemical constituent;structure identification

Abstract:

Keywords:

基金项目(Foundation): 国家“863”项目(2007AA09Z413,2007AA09Z435);; 国家自然科学基金(40776098,20602009)

作者(Authors): 王彧博;郑立;李文;裴月湖;
WANG Yu-bo1,ZHENG Li2,LI Wen3,PEI Yue-hu1(1.School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China;2.Qingdao Key Laboratory of Analytical Technology Development and Standardization of Chinese Medicines,First Institute of Oceanography,State Oceanic Administration,Qingdao 266061,China;3.The Test Center for Analysis,Shenyang Pharmaceutical University,Shenyang 110016,China)

DOI: 10.14066/j.cnki.cn21-1349/r.2010.05.020

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