罗媛,杨武,孙慧园,孙佳,董莉,陈思颖,黄勇
LUO Yuan,YANG Wu,SUN Hui-yuan,SUN Jia,DONG Li,CHEN Si-ying,HUANG Yong

• 1:贵州医科大学贵州省药物制剂重点实验室
• 2:贵州省骨科医院药剂科
• 3:贵州医科大学药学院

摘要(Abstract)：

目的研究大鼠肠道菌群对没食子酸和原儿茶酸2个单体成分的代谢作用。方法建立UHPLC-Q-TOF/MS检测条件,流动相为体积分数0.1%甲酸水溶液(A)-体积分数0.1%甲酸乙腈溶液(B)梯度洗脱,流速为0.3 m L·min-1,扫描方式为电喷雾(ESI)负离子模式。采用负离子和多反应离子监测模式分析没食子酸和原儿茶酸分别与大鼠肠道菌群共孵育后的代谢产物。结果从药物菌群孵育液中鉴定出没食子酸和原儿茶酸在大鼠肠道细菌各种酶的作用下发生脱羧和甲基化反应。结论阐明了没食子酸和原儿茶酸在大鼠肠道菌群中的代谢特征。
Objective To investigate the metabolism of gallic acid and protocatechuic acid in rat intestinal flora on.Methods UHPLC-Q-TOF / MS detection conditions was established with a mobile phase composed of 0.1% formic acid in water(A) and 0.1% formic acid in acetonitrile(B) in gradient mode at a flowrate of0.3 m L·min- 1.Negative ion and multiple reaction monitoring(MRM) mode was applied to analyze the metabolites of two components in rat intestinal flora.Results The metabolic reaction of gallic acid and protocatechuic acid was decarboxylation and methylation respectively.Conclusions The in vitro metabolic characteristics of gallic acid and protocatechuic acid by rat intestinal flora have been elucidated.

关键词(KeyWords)： 肠道菌群;没食子酸;原儿茶酸;UHPLC-Q-TOF/MS;代谢产物
intestinal flora;gallic acid;protocatechuic acid;UHPLC-Q-TOF/MS;metabolism

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(81260688);; 贵州省研究生卓越人才计划项目(黔教研合ZYRC字[2014]012);; 黔科合人字(2015)11号;; 黔人领发[2013]15号(8)

作者(Author): 罗媛,杨武,孙慧园,孙佳,董莉,陈思颖,黄勇
LUO Yuan,YANG Wu,SUN Hui-yuan,SUN Jia,DONG Li,CHEN Si-ying,HUANG Yong

DOI: 10.14066/j.cnki.cn21-1349/r.2017.01.008

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