荆蕴杰,方亮,郭汉文
JING Yun-jie1,FANG Liang1,GUO Han-wen2(1.School of Pharmacy

• 1:沈阳药科大学药学院
• 2:辽宁省食品药品检验所

摘要(Abstract)：

目的研究复方脚癣灵的体外经皮渗透特征,确定最佳促进剂及浓度。方法以自制双室水平扩散池和离体鼠皮进行体外渗透实验,采用HPLC法同时测定盐酸小檗碱和黄芩苷的皮内含量。结果促进剂为丙二醇和薄荷醇,最佳质量分数均为3%时,复方脚癣灵中2种成分的皮内蓄积量有显著的提高。结论应用促进剂后,制剂中小檗碱与黄芩苷在离体鼠皮内有较强的蓄积作用。
Objective To investigate the effects of penetration enhancers on the percutaneous absorption of compound Jiaoxuanling linimentum(traditional Chinese medicine) and selected the optimal concentration of enhancers.Methods Permeations studies were carried out in vitro across rat skin by side-by-side glass diffusion cells.The retention amounts of baicalin and berberine in excised rat skin were simultaneously determined by HPLC method.Results Combination use of 3% PG and 3% menthol provided the highest increase of accumulation of baicalin and berberine in the excised rat skin.Conclusions Epidermal retention of baicalin and berberine is significantly increased after application of enhancers.

关键词(KeyWords)： 复方脚癣灵搽剂;经皮吸收;滞留量;促进剂;盐酸小檗碱;黄芩苷
compound Jiaoxuanling linimentum;percutaneous absorption;retention amount;enhancer;berberine hydrochioride;baicalin

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 荆蕴杰,方亮,郭汉文
JING Yun-jie1,FANG Liang1,GUO Han-wen2(1.School of Pharmacy

参考文献(References)：

• [1]荆蕴杰,黄葳薇,方亮,等.正交试验优选复方脚癣灵提取工艺[J].中华中医药学刊,2008,26(3):655-656.
• [2]杨得坡,胡海燕,黄世亮.黄芩苷元和黄芩苷对皮肤真菌与细菌抑制作用的研究[J].中药材,2000,23(5):272-274.
• [3]刘春平,赵淑肖,陈强,等.小檗碱抗5种皮肤癣菌实验观察[J].临床皮肤科杂志,2005,34(1):29.
• [4]MEGRAB N A,WILLIAMS A C,BARRY B W.Oestradiol permeation across human skin and snake skinmenbranes:the effects of ethanol/water co-solvent sys-tems[J].Int J Pharm,1995,116(1):101-112.
• [5]THOMAS N S,PANCHAGNULA R.Transdermal de-livery of zidovudine:Effect of vehicles on permeationacross rat skin and their mechanism of action[J].Eur JPharm Sci,2003,18(1):71-79.
• [6]郑俊民.经皮给药新剂型[M].北京:人民卫生出版社,2006:84.