乔巧玉,刘峻男,邱昭昭,赵旻,王淼,赵春杰
QIAO Qiaoyu,LIU Junnan,QIU Zhaozhao,ZHAO Min,WANG Miao,ZHAO Chunjie

• 1:沈阳药科大学药学院
• 2:沈阳药科大学生命科学与生物制药学院

摘要(Abstract)：

目的 建立HPLC法测定菊明降压丸中五种成分含量的方法。方法 采用Kromat Universil XB-C_(18)色谱柱(150 mm×2.1 mm, 3μm),以乙腈-体积分数为0.1%磷酸水为流动相，在流速为0.2 mL·min~(-1),柱温为30℃,检测波长为284 nm和337 nm的条件下进行梯度洗脱，对其中的绿原酸、蒙花苷、木犀草素、芹菜素、橙黄决明素的含量进行测定。结果 绿原酸、蒙花苷、木犀草素、芹菜素、橙黄决明素的质量浓度分别在20.9～334.1、3.7～59.1、7.0～111.9、0.7～11.9和2.1～33.5 mg·L~(-1)内与各自峰面积呈良好的线性关系，平均回收率分别为100.6%、99.9%、101.2%、98.6%和98.8%,RSD分别为1.5%、1.2%、1.1%、1.4%和1.0%(n=9)。结论 重复性好，可为菊明降压丸质量控制提供科学的参考。
Objective To establish a high performance liquid chromatography method for simultaneous determination of five components in Juming Jiangya Wan.Methods Gradient elution was performed using a Kromat Universil XB-C_(18) column(150 mm×2.1 mm, 3.0 μm).The mobile phase was acetonitrile-0.1% phosphoric acid, the flow rate was 0.2 mL·min~(-1),the detection wavelengths were 284 nm and 337 nm, and the column temperature was set at 30 ℃.The contents of chlorogenic acid, buddleoside, luteolin, apigenin, aurantio-obtusin were measured.Results The mass concentrations of chlorogenic acid, buddleoside, luteolin, apigenin, aurantio-obtusin had a good linear relationship with their respective peak areas at 20.9-334.1,3.7-59.1,7.0-111.9,0.7-11.9 and 2.1-33.5 mg·L~(-1).The average recoveries were 100.6%,99.9%,101.2%,98.6% and 98.8%.The RSD were 1.5%,1.2%,1.1%,1.4% and 1.0%(n=9).Conclusion This method can provide scientific reference for the quality control of Juming Jiangya Wan.

关键词(KeyWords)： 菊明降压丸;高效液相色谱法;含量测定
Juming Jiangya Wan;high performance liquid chromatography;content determination

Abstract:

Keywords:

基金项目(Foundation): 辽宁省教育厅项目(2017LQN19)

作者(Author): 乔巧玉,刘峻男,邱昭昭,赵旻,王淼,赵春杰
QIAO Qiaoyu,LIU Junnan,QIU Zhaozhao,ZHAO Min,WANG Miao,ZHAO Chunjie

DOI: 10.14066/j.cnki.cn21-1349/r.2021.0021

参考文献(References)：

• [1] LIU L S.Chinese guidelines for the prevention and treatment of hypertension,revised in 2018[J].Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases(心脑血管病防治),2019,19 (1):1-44.
• [2] Chinese Center for Disease Control and Prevention,Chinese Center for Disease Control and Prevention of chronic and noncommunicable diseases.Monitoring report on chronic diseases and risk factors in China-2010(中国慢性病及其危险因素监测报告)[M].Beijing:Military Medical Science Press,2012:233.
• [3] WANG X,LU J.Analysis on the use of antihypertensive drugs in 6 hospitals in Xianyang city[J].Anhui Medicine(安徽医药),2017,21(4):747-751.
• [4] CAO B M,ZHOU Y L,LI Y S,et al.The prevalence of hypertension and the risk analysis of overall cardiovascular events in community residents over 35 years old[J].Modern Medicine(现代医学),2011,39(5):529-532.
• [5] Chinese Pharmacopoeia Commission.Pharmaceutical standards of the Ministry of Health of the People′s Republic of China Prescriptions of traditional Chinese medicines:Volume Three(中华人民共和国卫生部药品标准中药成方制剂：第三册)[S].Beijing:Pharmacopoeia Committee of the Ministry of Health of the People′s Republic of China,1991:64.
• [6] WU X S,XU J,ZHANG T J,et al.Research progress on the chemical composition and quality evaluation of wild chrysanthemum[J].Chinese Herbal Medicine(中草药),2015,46(3):443-452.
• [7] ZHENG L L,ZHANG G J,WANG J J,et al.Study on anti-inflammatory biological effects of active components of chrysanthemum[J].Tianjin Traditional Chinese Medicine(天津中医药),2011,28(3):251-253.
• [8] ZHANG A L,MA Q,GAO J M,et al.Original acid and its analogues and biological activity[J].Chinese Herbal Medicine(中草药),2001(02):79-82.
• [9] ZHANG J Y,ZHANG L,JIN Y,et al.Anti-inflammatory effect and partial mechanism of total flavonoids of wild chrysanthemum[J].Journal of Anhui Medical University(安徽医科大学学报),2005(5):405-408.
• [10] CHEN K,QU Y N.Research progress on active ingredients and application of wild chrysanthemum[J].Anhui Chemical Industry(安徽化工),2014,40(1):10-13.
• [11] LIU J F,ZHU Q Z,QIAN M K,et al.Study on drugs for treatment of hypertension ⅩⅢ.Experimental treatment and toxicity of HC-1 from wild chrysanthemum[J].Acta Pharmaceutica Sinica(药学学报),1962(3):151-154.
• [12] LI G D,CHEN Y Y,WANG P,et al.Study on the hepatoprotective effects of terpenoids and flavonoids in wild chrysanthemum [J].Chinese Herbal Medicine(中草药),2013,44(24):3510-3514.
• [13] CHEN Y Y.Study on the synergistic effects of terpenoids and flavonoids in wild chrysanthemum on immune regulation and liver protection(野菊花中萜类、黄酮类化合物对免疫调节和肝保护的协同作用研究)[D].Zhengzhou:Zhengzhou University,2012.
• [14] CHU F H,YU X.Study on the scavenging effect of wild chrysanthemum extract on free radicals[J].Food Industry(食品工业),2010,31(5):1-3.
• [15] ZHANG Z Y,FANG X P,DIAO Z H,et al.In vitro experimental study on the inhibitory effect of wild chrysanthemum extract on respiratory syncytial virus[J].Pharmaceutical Journal of Chinese People′s Liberation Army(解放军药学学报),2006(4):273-276.
• [16] HU Z J,WANG L,ZHANG J X,et al.Study on the identification of Cassia seeds by thin layer chromatography[J].Lishizhen Medicine and Materia Medica(时珍国医国药),2006(11):2129.
• [17] ZHANG Y,HUANG X P,WENG D Q,et al.Determination of anthraquinones in Cassia seeds from different places by HPLC[J].Chinese Journal of Traditional Chinese Medicine(中国中药杂志),2008,33(23):2797-2799.
• [18] HAN C Z.The eyesight improving effect of cassia seed[J].Chinese Journal of Hospital Pharmacy(中国医院药学杂志),1993,13(5):200-201.
• [19] PATIL U K,SARAF S,DIXIT V K.Hypolipidemic activity of seeds of Cassia tora Linn[J].Journal of Ethnopharmacology,2004,90(2/3):249-252.
• [20] LIN Z H,XIONG Y.Effect of Cassia seed extract on blood lipid in hyperlipidemic rat model[J].New Chinese Medicine(新中医),2010,42(7):111-112.
• [21] TANG Y J.Experimental study on the antihypertensive effect of Cassia seed water extract on spontaneously hypertensive rats[J].Chinese and Foreign Medical Research(中外医学研究),2012,10(27):141.
• [22] ZHOU L.Protective effect of Cassia seed water extract on acute liver injury in mice[J].Sichuan Traditional Chinese Medicine(四川中医),2010,28(12):56-57.
• [23] QING J J.Observation on the curative effect of Cassia seed in the treatment of prehypertension[J].Chinese Folk Therapy(中国民间疗法),2010,18(6):26.
• [24] WANG G Y.Analysis of the clinical efficacy of Cassia seed in the treatment of senile constipation[J].Chinese Community Physician (Medical Major)[中国社区医师(医学专业)],2012,14(24):188.
• [25] LU H X,QIAO Y L,LIANG C H,et al.Determination of mongolian anthocyanin in Juming Jiangya Wan by HPLC[J].Chinese Herbal Medicine(中草药),2010,41(12):2002-2003.
• [26] GUO T,ZUO C X,CAO K Q.Discussion on the determination method of cassia seed in the 2015 edition of Chinese Pharmacopoeia[J].Journal of shanxi University of Traditional Chinese Medicine(陕西中医药大学学报),2017,40(6):118-120,123.