GC和GC-MS法测定注射用大豆油中脂肪酸组成及含量Determination of the contents of four fatty acids in soybean oil for injection by GC-MS and GC
高艺恬,赵丽梅,盛明,王淼,赵春杰
GAO Yi-tian,ZHAO Li-mei,SHENG Ming,WANG Miao,ZHAO Chun-jie
摘要(Abstract)：
目的分析注射用大豆油中主要脂肪酸组成,并建立同时测定大豆油中棕榈酸、硬脂酸、油酸和亚油酸4种成分含量的方法。方法采用GC-MS法确定大豆油中4种主要脂肪酸组成,采用毛细管气相色谱法测定甲酯化后大豆油中棕榈酸、硬脂酸、油酸和亚油酸的含量。结果棕榈酸甲酯、硬脂酸甲酯、油酸甲酯和亚油酸甲酯的线性范围分别为0.508 0~8.128g·L-1(r=0.999 0)、0.126 0~2.016g·L-1(r=0.999 4)、1.007~16.11g·L-1(r=0.999 4)和2.522~40.34g·L-1(r=0.999 4);各成分的平均回收率分别为(100.8±0.64)%、(101.6±0.89)%、(100.5±0.31)%和(99.4±0.84)%,RSD分别为1.36%、0.68%、1.11%和1.03%(n=9)。结论确定了4种主要脂肪酸组成,建立了采用内标法对注射用大豆油中4种脂肪酸进行含量测定,该方法准确可靠,可为注射用大豆油的质量评价提供依据。
Objective To develop a method for the determination of palmitic acid,octadecanoic acid,oleic acid and linoleic acid in soybean oil for injection simultaneously.Methods Analyzed the composing of fatty acid of soybean oil for injection by GC-MS.Results The calibration curve was linear within the range of 0.508 0-8.128 g·L-1(r=0.999 0),0.126 0-2.016 g·L-1(r=0.999 4),1.00716.11 g·L-1(r=0.999 4)and2.522-40.34 g·L-1(r=0.999 4)for methyl hexadecanoate,methyl octadecanoate,methyl cis-9-octadecenoate,methyl linoleate,respectively.The average recoveries(n= 9)were(100.8±0.64)%,(101.6±0.89)%,(100.5± 0.31)% and(99.4 ±0.84)% for palmitic acid,octadecanoic acid,oleic acid and linoleic acid,respectively.Conclusions This method is accurate with the good repeatability and can be used for the quality control of soybean oil for injection.
关键词(KeyWords)：
大豆油(供注射用);GC-MS;GC;脂肪酸;含量测定
soybean oil;GC-MS;GC;fatty acid;content determination
基金项目(Foundation):
作者(Author):
高艺恬,赵丽梅,盛明,王淼,赵春杰
GAO Yi-tian,ZHAO Li-mei,SHENG Ming,WANG Miao,ZHAO Chun-jie
DOI: 10.14066/j.cnki.cn21-1349/r.2013.09.002
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