李宇,袁名远,阳沁,谭旭辉,郭汉玖,郭杰,冉亚兰,何美军
LI Yu,YUAN Mingyuan,YANG Qin,TAN Xuhui,GUO Hanjiu,GUO Jie,RAN Yalan,HE Meijun

• 1:湖北省农业科学院中药材研究所国家中药材产业技术体系恩施综合试验站
• 2:湖北民族大学生物科学与技术学院
• 3:湖北星斗山国家级自然保护区管理局
• 4:恩施州农业农村局

摘要(Abstract)：

目的 研究过热蒸汽干燥和热风干燥两种干燥工艺对恩施地道药材湖北贝母花挥发性成分和抑菌活性影响。方法 通过GC-MS技术分析两种干燥方式处理的贝母花挥发成分，并对10株供试菌进行体外抑菌活性研究。结果 从过热蒸汽干燥处理组中鉴定出33个化合物，占相对百分含量12.36%,热风干燥处理组中鉴定出28个化合物，占相对百分含量7.14%,两组相同化合物23个；羧酸类和醇类化合物为主要挥发性成分，其含量在过热蒸汽干燥处理组和热风干燥处理组中分别为7.09%、3.43%;过热蒸汽干燥处理组对肺炎克雷伯菌、金黄色葡萄球菌、粪链球菌、耐甲氧西林金黄色葡萄球菌、多耐铜绿假单胞菌、溶藻弧菌以及表皮葡萄球菌等7种供试菌有较好抑制效果，热风干燥处理组对肺炎克雷伯菌、苏云金芽孢杆菌、多耐铜绿假单胞菌以及表皮葡萄球菌等4种供试菌有一定抑制效果。结论 两种干燥方式对湖北贝母花的挥发性成分组成、含量和抑菌活性存在差异，且过热蒸汽干燥组的抑菌活性优于热风干燥组。该结果为湖北贝母花的开发利用提供了科学依据。
Objective To research the effects of two drying process superheated steam drying and hot air drying on the flowers of Fritillaria Hupehensis Bulbus, genuine medicinal materials from Enshi.Methods The GC-MS technology was used to analyze the differences of volatile components in flowers of F.hupehensis treated by two drying methods.And the bacteriostatic activity of 10 strains in vitro was tested.Results 33 compounds were identified from the superheated steam drying group, accounting for 12.36% of the relative content.28 compounds were identified from the hot air drying group, accounting for 7.14% of the relative content.And there were 23 identical compounds in the two groups, which were the main volatile components carboxylic acid and alcohol compounds and contents of which were 7.09% in the superheated steam drying group and 3.43% in the hot air drying group, respectively.Superheat steam drying group had good inhibitory effect on 7 strains, including Klebsiella pneumonia ATCC 13883,Staphylococcus aureus ATCC 29213,Enterococcus faecalis ATCC 29212,Methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, Vibrio alginolyticus XSBZ14 and methicillin-resistant Staphylococcus epidermidis.The hot air drying group had weak inhibitory effect on 4 strains, including Klebsiella pneumonia ATCC 13883,Bacillus thuringiensis, Pseudomonas aeruginosa and methicillin-resistant Staphylo-coccus epidermidis.Conclusion There were differences in the composition, content and bacteriostatic activities of volatile components in the two groups.And the bacteriostatic activity of superheated steam drying group was better than of hot air drying group.These results provided a scientific basis for the development and utilization of flowers of F.hupehensis.

关键词(KeyWords)： 过热蒸汽干燥;热风干燥;湖北贝母花;挥发性成分;抑菌活性
superheat steam drying;hot air drying;flowers of Fritillaria Hupehensis Bulbus;volatile component;bacteriostatic activity

Abstract:

Keywords:

基金项目(Foundation): 湖北省重点研发计划“道地药材黄连、湖北贝母新品种培育及关键技术研究”资助项目(2020BCA059);; 湖北省现代农业产业技术体系“道地药材提质增效生产技术集成创新与示范”资助项目(HBHZD-ZB-2020-005)

作者(Author): 李宇,袁名远,阳沁,谭旭辉,郭汉玖,郭杰,冉亚兰,何美军
LI Yu,YUAN Mingyuan,YANG Qin,TAN Xuhui,GUO Hanjiu,GUO Jie,RAN Yalan,HE Meijun

DOI: 10.14066/j.cnki.cn21-1349/r.2021.0303

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