胶束薄层色谱法同时测定APC片中3组分含量Simultaneous determination of the contents of the three components in the APC by micellar thin layer chromatography
赵怀清,王学娅,杨丽,程丽珠
ZHAO Huai qing 1; WANG Xue ya 2; YANG Li 3; CHENG Li zhu 1 (1. College of Pharmacy;
摘要(Abstract)：
应用胶束薄层色谱法同时测定复方乙酰水杨酸片 (APC)中的阿司匹林 (A)、非那西汀 (P)和咖啡因 (C)的含量。在聚酰胺薄膜上 ,以 2 %SDS 乙腈 NaAc Na2 HPO4 ·1 2H2 O(3∶1∶1 )为展开剂 ,测定波长为 2 73nm ,单波长反射法锯齿扫描 ,结果 ,点样量在 0 9～ 4 5 μg,A组分的点样量X(mi)与斑点积分面积的比值Y(Ai/As)呈良好的线性关系 ,其回归方程为 :Y =2 0 0 3 1X +0 81 5 9,r =0 9981 ,平均回收率为 1 0 1 3 % ,RSD 1 9% ;点样量在 0 6～ 3 0 μg,P组分的回归方程为 :Y =1 2 1 4 5X +0 4 1 0 6,r=0 9978,平均回收率为 99 2 % ,RSD 2 4 % ;点样量在 0 1 4～ 0 7μg,C组分的回归方程为 :Y =2 1 3 63X +0 64 5 2 ,r=0 9984 ,平均回收率为 :98 4 % ,RSD 3 1 %
A sensitive method was developed to simultaneous determination of the contents of aspirin (A),phanacetin (P) and caffein (C) in the APC tablet by micellar thin layer chromatography. The developing solvent was 2% sodium dodecyl sulfate/sodium acetate disodiun hydrogen phosphate (pH8) /acetonitrile (3∶1∶1) . The UV detector was set at 273 nm .The calibration curves were linear in the range of 0 9～ 4 5 μg for A, 0 6～ 3 0 μg for P, 0 14～ 0 7 μg for C. The regression equations were Y= 2 003 1X + 0 815 9 , r = 0 998 1 for A, Y = 1 214 5 X + 0 410 6 , r = 0 997 8 for P, Y = 2 136 3 X + 0 645 2 , r =0 998 4 for C. The average recoveries for A, P and C were 101 3%(RSD 1 9), 99 2%(RSD 2 4)and 98 4%(RSD 3 1)respectively. The method is very simple, rapid and the results are quite satisfactory.
关键词(KeyWords)：
胶束薄层色谱;阿司匹林;非那西汀;咖啡因
aspirin; phanacetin; caffein; micellar thin layer chromatography
基金项目(Foundation):
作者(Authors):
赵怀清,王学娅,杨丽,程丽珠
ZHAO Huai qing 1; WANG Xue ya 2; YANG Li 3; CHENG Li zhu 1 (1. College of Pharmacy;
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