李宝媛,战凯旋,周雨华,郭远强,回业乾,李玉山
LI Bao-yuan1,ZHAN Kai-xuan1,ZHOU Yu-hua1,GUO Yuan-qiang2,HUI Ye-qian3,LI Yu-shan1(1.School of Traditional Chinese Materia Medica

• 1:沈阳药科大学中药学院
• 2:南开大学药学院
• 3:沈阳腾晔生物药业科技研发有限公司

摘要(Abstract)：

目的对蒙药沙蓬的化学成分进行研究。方法采用大孔树脂柱色谱、反复硅胶柱色谱、Seph-adex LH-20柱色谱、ODS柱色谱和半微量制备高效液相色谱分离纯化,根据ESI-MS、1H-NMR和13C-NMR等谱学数据进行结构鉴定。结果从沙蓬全草的乙醇提取物的正丁醇萃取物和乙酸乙酯萃取物中分离得到7个黄酮类和2个香豆素类化合物,分别鉴定为芦丁(quercetin-3-O-rutinoside,1)、异鼠李糖-3-O-β-D-芸香糖苷(isorhamnetin 3-O-β-D-rutinoside,2)、tamarixetin 3-O-rutinoside(3)、槲皮素-3-O-β-D-吡喃葡萄糖苷(quercetin 3-O-β-D-glucopyranoside,4)、异鼠李素-3-葡萄糖苷(isorh-amnetin-3-glucoside,5)、二氢木蝴蝶素A(dihydrooroxylin A,6)、5,2'-二羟基-6,7-亚甲二氧基二氢黄酮(5,2'-dihydroxy-6,7-methylenedioxyflavanone,7)、异莨菪亭(isoscopoletin,8)和东莨菪亭(scopoletin,9)。结论化合物3、6、7为首次从藜科植物中分离得到,化合物1、2、4、5、8、9为首次从沙蓬属植物中分离得到。
Objective To isolate the constituents of the Mongolian medicine-Agriophyllum squarrosum(L.)Moq.and elucidate their structures.Methods The compounds were isolated by macroporous resin,silica gel,Sephadex LH-20,ODS,and semipreparation.HPLC column chromatography,and the ESI-MS,1H-NMR,13C-NMR spectroscopic analysis were employed for the structural elucidation.Results Seven flavonoiols and two coumarins were isolated from the n-BuOH extract and EtOAc extract of the aerial parts of Agriophyllum squarrosum(L.)Moq.and their structures were identified as quercetin-3-O-rutinoside(1),isorhamnetin 3-O-β-D-rutinoside(2),tamarixetin 3-O-rutinoside(3),quercetin 3-O-β-D-glucopyranoside(4),isorhamnetin 3-glucoside(5),dihydrooroxylin A(6),5,2′-dihydroxy-6,7-methylenedioxyflavanone(7),isoscopoletin(8)and scopoletin(9).Conclusions Compounds 3,6 and 7 are isolated from the family Chenopodiaceae for the first time and compounds 1,2,4,5,8,9 are isolated from the genus Agriophyllum for the first time.

关键词(KeyWords)： 沙蓬;黄酮类;香豆素类;化学成分;结构鉴定
Agriophyllum squarrosum;flavonoid;coumarin;chemical constituent;structure identification

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 李宝媛,战凯旋,周雨华,郭远强,回业乾,李玉山
LI Bao-yuan1,ZHAN Kai-xuan1,ZHOU Yu-hua1,GUO Yuan-qiang2,HUI Ye-qian3,LI Yu-shan1(1.School of Traditional Chinese Materia Medica

DOI: 10.14066/j.cnki.cn21-1349/r.2012.12.003

参考文献(References)：

• [1]内蒙古植物志编辑委员会.内蒙古植物志(第2版):第2卷[M].呼和浩特:内蒙古人民出版社,1998:277.
• [2]朱亚民.内蒙古植物药志:第1卷[M].呼和浩特:内蒙古人民出版社,2000:299.
• [3]国家中医药管理局中华本草编委会.中华本草:蒙药分卷[M].上海:上海科学技术出版社,2000:236.
• [4]斯建勇,陈迪华,高光跃.云南山楂叶化学成分的研究[J].中国中药杂志,1998,23(7):422-423.
• [5]GUTZEIT D,WRAY V,WINTERHALTER P,et al.Preparative isolation and purification of flavonoids andprotocatechuic acid from sea buckthorn juice concen-trate(Hippophae rhamnoides L.ssp.rhamnoides)byhigh-speed counter-current[J].Chromatography,2007,65(1/2):1-7.
• [6]XU X Y,XIE H H,HAO J,et al.Flaronoid glycosidesfrom the seeds of Litchi chinensis[J].Journal of Agri-cultural and Food Chemistry,2011,59:1205-1209.
• [7]KIM J Y,CHO J Y,MA Y K,et al.Dicaffeoylquinicacid derivatives and flavonoid glucosides from glass-w ort(Salicornia herbacea L.)and their antioxidativeactivity[J].Food Chemistry,2011,125:55-62.
• [8]DA ROSA E A,E SILVA B C,DA SILVA F M,etal.Flavonoides e atividade antioxidante em Palicourearigida Kunth.,Rubiaceae[J].Revista Brasileira deFarmacognosia Brazilian Journal of Pharmacognosy,2010,20(4):484-488.
• [9]肖丽和,王红燕,宋少江,等.滇黄芩化学成分的分离与鉴定[J].沈阳药科大学学报,2003,20(3):181-183.
• [10]KOJIMA K,GOMBOSURENGYIN P,ONDOGNYIP,et al.Flavanones from Iris tenuifolia[J].Phyto-chemistry,1997,44(4):711-714.
• [11]KIM B G,LEE Y,HUR H,et al.Production of threeO-methhylated esculetins w ith Escherichia coli Ex-pressing O-methyltransferase from poplar[J].BiosciBiotechnol Biochem,2006,70(5):1269-1272.