梁艳芬,黄鸿章,许丽媛,黎亮星,李宁
LIANG Yan-fen,HUANG Hong-zhang,XU Li-yuan,LI Liang-xing,LI Ning

• 1:广东药学院药科学院

摘要(Abstract)：

目的建立用于同时测定大鼠血浆中丹参素钠、迷迭香酸和丹酚酸B的HPLC法,并研究丹参水溶性提取物在大鼠体内的药动学特征。方法大鼠灌胃给予丹参水溶性提取物(丹参素钠78.1 mg·kg-1、迷迭香酸40.4 mg·kg-1、丹酚酸B1.2 g·kg-1)后,眼眶静脉丛取血,经盐酸酸化及乙酸乙酯萃取后采用HPLC测定不同时间点的血药浓度。结果丹参素钠、迷迭香酸和丹酚酸B分别在0.4~10.0、0.24~6.00、0.8~10.0 mg·L-1内线性良好,方法回收率均在85%~115%之间,日内、日间精密度良好,稳定性符合要求。主要药动学参数t1/2(ka)分别为0.052、0.172、0.080 h;ρmax分别为1.072、2.290、4.128 mg·L-1;AUCo-t分别为6.674、6.933、8.943 mg·h·L-1。结论丹参素钠在大鼠体内的药动学过程符合单室模型,迷迭香酸和丹酚酸B符合二室模型。
Objective To establish a high-performance liquid chromatography for the simultaneous determination of sodium danshensu,rosmarinic acid and salvianolic acid B in rat plasma and study the pharmacokinetic characteristics of the three compounds. Methods The rats were given the extract of salvia miltiorrhiza( 78. 1 mg·kg- 1sodium danshensu,40. 4 mg·kg- 1rosmarinic acid and 1. 2 g·kg- 1salvianolic acid B) by oral administration,and the blood samples were collected from eye ophthalmic vein jungle venous plexus. After precipitated by 10% HCl and extracted by ethyl acetate,plasma concentrations of sodium danshensu,rosmarinic acid and salvianolic acid B were determined by HPLC. Results The linear ranges of sodium danshensu,rosmarinic acid and salvianolic acid B were from 0. 4 to 10. 0 mg·L- 1,0. 24 to 6. 00 mg·L- 1and 0. 8 to10. 0 mg·L- 1,respectively. Good precision and stability were showed and the average recoveries were all between( 100 ± 15) %. The pharmacokinetic parameters of the three compounds were as follow: t1 /2( ka)were 0. 052,0. 172 and 0. 080 h,ρmaxwere 1. 072,2. 290 and 4. 128 mg·L- 1,AUCo-twere 6. 674,6. 933 and8. 943 mg·h·L- 1. Conclusions The pharmacokinetic process of sodium danshensu fits to one compartment models and the pharmacokinetic processes of rosmarinic acid and salvianolic acid B fit to two-compartment models.

关键词(KeyWords)： 丹参水溶性提取物;丹参素钠;迷迭香酸;丹酚酸B;高效液相色谱法;药动学
the aqueous extracts of salvia miltiorrhiza;sodium danshensu;rosmarinic acid;salvianolic acid B;HPLC

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(81173525)

作者(Author): 梁艳芬,黄鸿章,许丽媛,黎亮星,李宁
LIANG Yan-fen,HUANG Hong-zhang,XU Li-yuan,LI Liang-xing,LI Ning

DOI: 10.14066/j.cnki.cn21-1349/r.2015.11.009

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