高婷;曹阳;贾凌云;丁宏;孙启时;
GAO Ting1,CAO Yang2,JIA Ling-yun1,DING Hong1,SUN Qi-shi1(1.School of Traditional Chinese Materia Medic,Shenyang Pharmaceutical University,Shenyang 110016,China;2.Shenyang Huisheng Pharmaceutical Co.,Ltd.,Shenyang 110001,China)

• 1:沈阳药科大学中药学院
• 2:沈阳惠生药业有限公司

摘要(Abstract)：

目的建立测定山楂中羽扇豆醇含量的方法。方法采用HPLC法。色谱柱为Diamonsil C18柱(200 mm×4.6 mm,5μm),流动相为甲醇-水(体积比60∶40),流速为1.0 mL.min-1,检测波长为205 nm,柱温为25℃,进样量为10μL。结果羽扇豆醇质量浓度在0.432⁴.32 mg.L-1内与峰面积呈良好的线性关系,r=0.999 8(n=5),平均回收率为95.5%(RSD=3.2%,n=9)。结论该方法简便,准确,重现性好,可用于山楂药材的质量评价。
Objective To establish an HPLC method for the determination of lupeol in Fructus Crataegi.Methods Chromatography was conducted on a Diamonsil C18 column(200 mm×4.6 mm,5 μm).The mobile phase was methanol-water(V∶V=60∶40)with a flow rate of 1.0 mL·min-1 and the detection wavelength was set at 205 nm,column temperature at 25 ℃.Results The calibration curve was linear in the range of 0.432-4.32 mg·L-1(r=0.999 8).The average recovery was 95.5% and RSD was 3.2%(n=9).Conclusions The method is simple,accurate,reproducible,and can be used to evaluate the quality of Fructus Crataegi.

关键词(KeyWords)： 山楂;羽扇豆醇;高效液相色谱法
Fructus Crataegi;lupeol;HPLC

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 高婷;曹阳;贾凌云;丁宏;孙启时;
GAO Ting1,CAO Yang2,JIA Ling-yun1,DING Hong1,SUN Qi-shi1(1.School of Traditional Chinese Materia Medic,Shenyang Pharmaceutical University,Shenyang 110016,China;2.Shenyang Huisheng Pharmaceutical Co.,Ltd.,Shenyang 110001,China)

DOI: 10.14066/j.cnki.cn21-1349/r.2010.05.006

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