张帅男,李红美,李煦照
ZHANG Shuainan,LI Hongmei,LI Xuzhao

• 1:贵州中医药大学药学院

摘要(Abstract)：

目的 代谢组学、蛋白质组学和生物信息学被用于分析山豆根脑毒性的作用机制及物质基础。方法 运用代谢组学和蛋白质组学分析山豆根干预脑组织后的差异表达代谢物和蛋白质。随后，对这些内源性靶点在MetaboAnalyst数据库中进行联合通路分析。STITCH数据库和STRING数据库被用于筛选响应毒性靶点的物质基础。结果 组学结果表明，经山豆根干预后，小鼠脑组织中的38个代谢物和293个蛋白质发生差异表达。其中，11个差异表达的代谢物和21个差异表达的蛋白质参与了8个显著调节通路。生物信息学表明，24个成分有可能干预了18个毒性靶点的表达。结论 多个靶点参与了山豆根诱导的脑毒性，它们的异常表达有诱导神经兴奋性毒性、智力迟钝、记忆下降、皮质损伤等发生的可能性。多种成分有可能成为响应这些作用的物质基础。
Objective Metabolomics, proteomics, and bioinformatics were used to analyze the mechanism and material basis of the brain toxicity induced by Sophorae Tonkinensis radix et rhizome(ST).Methods Metabonomics and proteomics were applied to analyze the differentially expressed metabolites and proteins in brain tissue after treatment with ST.Subsequently, the endogenous targets were used for joint pathway analysis in MetaboAnalyst.STITCH and STRING database were applied to screen the material basis responsible for the toxic targets.Results Omics revealed that 38 metabolites and 293 proteins were differentially expressed after the treatment with ST,out of which 11 metabolites and 21 proteins were significantly enriched in 8 pathways.Bioinformatics showed that 24 compounds might interfere with the expressions of 18 toxic targets of ST.Conclusion Multiple targets are involved in the brain toxicity induced by ST,whose dysregulation may show the tendency to induce the occurrence of neuroexcitatory toxicity, mental retardation, memory decline, and cortical damage, etc.Multiple compounds may be the toxic material basis in response to these effects.

关键词(KeyWords)： 山豆根;脑毒性;代谢组学;蛋白质组学;生物信息学
Sophora tonkinensis Gagnep.;brain toxicity;metabonomics;proteomics;bioinformatics

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81960749);; 贵州中医药大学研究生教育创新计划重点项目(YCXZR2020002)

作者(Author): 张帅男,李红美,李煦照
ZHANG Shuainan,LI Hongmei,LI Xuzhao

DOI: 10.14066/j.cnki.cn21-1349/r.2021.0613

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