路橦;王侠;李佳鸿;林昱宏;李宁;孙璐;

• 1:沈阳药科大学药学院
• 2:国家林业和草原局文冠果工程技术研究中心
• 3:沈阳药科大学中药学院

摘要(Abstract)：

目的建立UPLC-QTOF-MS法同时测定春季文冠果叶中11种黄酮类化合物的含量。方法采用ACQUITY UPLC HSS T3色谱柱(100 mm×2.1 mm,1.8μm),以体积分数0.05%甲酸水为流动相A,体积分数0.05%甲酸乙腈为流动相B,梯度洗脱,流速0.4 mL·min~(-1),电喷雾离子源(ESI),负离子全扫描模式,选择性离子监测定量方式进行。结果表没食子儿茶素,表儿茶素,杨梅素-3-O-芸香糖苷,芦丁,杨梅苷,山柰酚-3-O-芸香糖苷,槲皮苷,杨梅树皮素,山柰酚-3-O-α-吡喃鼠李糖苷,木犀草素和槲皮素质量浓度分别在0.38～38.00、0.46～46.00、0.90～90.00、0.75～75.00、1.60～160.00、0.45～45.00、0.60～60.00、0.016～1.60、0.30～30.00、0.010～1.00、0.010～1.00 g·L~(-1)内与峰面积呈良好的线性关系,相关系数r均大于0.999。精密度、稳定性和重复性的RSD值均小于15%,平均回收率在91.5%～110.4%内,RSD小于6.5%。结论所建立的UPLC-QTOF-MS法适用于文冠果叶中黄酮类化合物的含量测定,为文冠果叶的合理应用提供依据。

关键词(KeyWords)： 文冠果叶;黄酮类化合物;含量测定;UPLC-QTOF-MS

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(81872768,81673323)

作者(Author): 路橦;王侠;李佳鸿;林昱宏;李宁;孙璐;

Email:

DOI: 10.14066/j.cnki.cn21-1349/r.2019.0555

参考文献(References)：

• [1] SHANG Q H,SUN YAN.Research progress on chemical constituents and pharmacological effects of Xanthoceras sorbifolia [J].Chinese Pharmacy (中国药房),2015,26(30):4316-4320.
• [2] Zhao C Y,Su H,Zhao Y H.Comprehensive development and utilization value of Xanthoceras sorbifolia [J].Forestry Survey and Design of Inner Mongolia(内蒙古林业调查设计),2008,31(6):118-122.
• [3] State Pharmacopoeia Commission.Pharmacopoeia of the People′s Republic of China:Volume I(中华人民共和国药典:一部) [M].Beijing:Chemical Industry Press,1977.121-122.
• [4] ZHANG Y S,GUO S Z,WEI Q,et al.Medicinal and health care efficacy and development and application of Xanthoceras sorbifolia [J].Guangzhou Chemical Industry(广州化工),2016,44 (1):46-55.
• [5] LUAN S N,HOU L Q,HUO L B,et al.Research and development of Xanthoceras sorbifolia resources in China(中国文冠果资源研究开发与实践)[M].Beijing:China Forestry Press,2007:612-613.
• [6] WANG P.Chemical constituents of Xanthoceras sorbifolia leaves(文冠果叶化学成分研究)[D].Xi′an:Shanxi University of Science and Technology,2010:15-37.
• [7] MA Y M,WANG P.Study on chemical constituents of Xanthoceras sorbifolia leaves [J].Chinese Patent Medicine(中成药),2010,32(10):1750-1755.
• [8] DA POZZO E,COSTA B,CAVALLINI C,et al.The citrus flavanone naringenin protects myocardial cells against age-associated damage[J].Oxid Med Cell Longev,2017,6(1):953-964.
• [9] JIMENEZ,LOPEZ-SEPULVEDA,ROMERO M,et al.Quercetin and its metabolites inhibit the membrane NADPH oxidase activity in vascular smooth muscle cells from normotensive and spontaneously hypertensive rats[J].Food Function,2015,6(2):409-414.
• [10] FANG H,PENG Z,WU H Y,et al.Inhibition of alpha-glucosidase and alpha-amylase by flavonoid glycosides from Lu′an GuaPian tea:molecular docking and interaction mechanism [J].Food Function,2018,9(9):4173-4183.
• [11] WEI J,ZHOU L W,HE J L,et al.Determination of six catechins in Oolong tea by HPLC [J].Analytical Instrument (分析仪器)2016,6:55-57.
• [12] CHEN T F,GAO W H,TANG M.Determination of dihydromyricetin and myricetin in ampelopsis grossedentata by HPLC [J].Asia Pacific Traditional Medicin(亚太传统医药) 201,1:1673-2197.
• [13] Huang P,he B,Liu Y,et al.Determination of four flavonoids in tartary buckwheat tea from different sources [J].Food Research and Development(食品研究与开发)2017,38( 1):135-138,1005-6521.
• [14] LYU C,CHEN L Q,FU P,et al.Simultaneous quantification of 11 active constituents in Shexiang Baoxin pill by ultraperformance convergence chromatography combined with tandem mass spectrometry[J].Journal of Chromatography B,2017,1052(12):135-141.
• [15] AN H J,WANG H,LAA Y X,et al.Simultaneous qualitative and quantitative analysis of phenolic acids and flavonoids for the quality control of Apocynum venetum L.leaves by HPLC DAD ESI IT TOF MS and HPLC DAD[J].Journal of Pharmaceutical and Biomedical Analysis,2013,85(5):295-304.
• [16] DONG R F,SU J,NIAN H,et al.Chemical fingerprint and quantitative analysis of flavonoids for quality control of sea buckthorn leaves by HPLC and UHPLC-ESI-QTOF-MS[J].Journal of Functional Foods,2017,37(2):513-522.
• [17] LYU X.J.,SUN Z,WANG P L,et al.Chemical profiling and quantification of Dan Deng Tong Nao capsule using ultra high-performance liquid chromatography coupled with high resolution hybrid quadruple orbitrap mass spectrometry[J].Journal of Pharmaceutical and Biomedical Analysis,2018,148(6):189-204.
• [18] LIANG J,WU W Y,SUN G X,et al.A dynamic multiple reaction monitoring method for the multiple components quantification of complex traditional Chinese medicine preparations:Niuhuang Shangqing pill as an example[J].Journal of Chromatography A,2013,1294(11):58-69.
• [19] WANG Q,SONG W,QIAO X,et al.Simultaneous quantification of 50 bioactive compounds of the traditional Chinese medicine formula Gegen-Qinlian decoction using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry[J].Journal of Chromatography A,2016,1454(8):15-25.
• [20] JIANG Y L,HUANG F,WU F H.Simultaneous determination of 16 flavonoids in Ginkgo health tea by HPLC-MS/MS [J].Chromatography(色谱)2015,33(10):1032～1039.
• [21] YI K,LIN Z C,LIU Q L,et al.Determination of three principal components in total flavonoids extract of Fujianese tea [J].Fujian Traditional Chinese Medicine,2017,48(2):51-55.