UPLC-QTOF-MS法同时测定春季文冠果叶中11种黄酮类化合物的含量Simultaneous determination of 11 flavonoids in spring leaves of Xanthoceras sorbifolia Bunge by UPLC-QTOF-MS
路橦;王侠;李佳鸿;林昱宏;李宁;孙璐;
摘要(Abstract):
目的建立UPLC-QTOF-MS法同时测定春季文冠果叶中11种黄酮类化合物的含量。方法采用ACQUITY UPLC HSS T3色谱柱(100 mm×2.1 mm,1.8μm),以体积分数0.05%甲酸水为流动相A,体积分数0.05%甲酸乙腈为流动相B,梯度洗脱,流速0.4 mL·min~(-1),电喷雾离子源(ESI),负离子全扫描模式,选择性离子监测定量方式进行。结果表没食子儿茶素,表儿茶素,杨梅素-3-O-芸香糖苷,芦丁,杨梅苷,山柰酚-3-O-芸香糖苷,槲皮苷,杨梅树皮素,山柰酚-3-O-α-吡喃鼠李糖苷,木犀草素和槲皮素质量浓度分别在0.38~38.00、0.46~46.00、0.90~90.00、0.75~75.00、1.60~160.00、0.45~45.00、0.60~60.00、0.016~1.60、0.30~30.00、0.010~1.00、0.010~1.00 g·L~(-1)内与峰面积呈良好的线性关系,相关系数r均大于0.999。精密度、稳定性和重复性的RSD值均小于15%,平均回收率在91.5%~110.4%内,RSD小于6.5%。结论所建立的UPLC-QTOF-MS法适用于文冠果叶中黄酮类化合物的含量测定,为文冠果叶的合理应用提供依据。
关键词(KeyWords): 文冠果叶;黄酮类化合物;含量测定;UPLC-QTOF-MS
基金项目(Foundation): 国家自然科学基金资助项目(81872768,81673323)
作者(Author): 路橦;王侠;李佳鸿;林昱宏;李宁;孙璐;
Email:
DOI: 10.14066/j.cnki.cn21-1349/r.2019.0555
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