赵静,姜■■■,张新,金东奎,孙秉华,游松
ZHAO Jing1,2,KANG Saeromi2,ZHANG Xin1,2,KIM Dong-kyoo2

• 1:沈阳药科大学生命科学与生物制药学院
• 2:韩国仁济大学生物药学化学学院
• 3:釜庆国立大学化学院

摘要(Abstract)：

目的对化合物2-(3E,5E-庚二烯基)-3,6-二羟基-5-(3-甲基-2-丁烯基)苯甲醛(isodihydroau-roglaucin)的抗癌活性进行研究。方法利用细胞毒实验测定isodihydroauroglaucin对人宫颈癌细胞(HeLa)的生长抑制作用;通过DNA含量分析和Annexin V-FITC/PI双染实验检测isodihydroauro-glaucin诱导的HeLa细胞死亡;利用单细胞凝胶电泳方法检测isodihydroauroglaucin对DNA的损伤作用。结果 Isodihydroauroglaucin对HeLa细胞的生长具有抑制作用,其IC50值为170μmol.L-1;DNA含量分析和Annexin V-FITC/PI双染实验表明isodihydroauroglaucin诱导HeLa细胞凋亡;isodi-hydroauroglaucin对DNA有损伤作用。结论 Isodihydroauroglaucin可能通过诱导DNA损伤诱导He-La细胞凋亡。
Objective To investigate the anticancer activity of 2-(3E,5E-heptadienyl)-3,6-dihydroxy-5-(3-methyl-2-butenyl)benzaldehyde(isodihydroauroglaucin). Methods Cytotoxicity assay was used to evaluate the anti-proliferation effect of isodihydroauroglaucin;DNA content analysis and Annexin V-FITC/PI dual staining assays were performed to determine the mode of HeLa cell death;single-cell gel electrophoresis was used to examine the DNA damage. Results Isodihydroauroglaucin inhibited HeLa cell growth,its IC50 value was about 170 μmol·L-1;results from DNA content analysis and Annexin V-FITC/PI dual staining assays showed that isodihydroauroglaucin induced apoptosis of HeLa cells;isodihydroauroglaucin induced DNA damage in HeLa cells. Conclusions Isodihydroauroglaucin may induce apoptotic cell death by inducing DNA damage.

关键词(KeyWords)： 2-(3E,5E-庚二烯基)-3,6-二羟基-5-(3-甲基-2-丁烯基)苯甲醛(isodihydroauroglaucin);细胞凋亡;DNA损伤;抗癌活性
isodihydroauroglaucin;apoptosis;DNA damage;anticancer activity

Abstract:

Keywords:

基金项目(Foundation): 韩国Brain Korea 21计划资助项目

作者(Author): 赵静,姜■■■,张新,金东奎,孙秉华,游松
ZHAO Jing1,2,KANG Saeromi2,ZHANG Xin1,2,KIM Dong-kyoo2

DOI: 10.14066/j.cnki.cn21-1349/r.2011.02.017

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