丰明凤,高岩,白嘉璇,尤丽娟,孙晓琳,王金辉,王立波,朴贤美
FENG Mingfeng,GAO Yan,BAI Jiaxuan,YOU Lijuan,SUN Xiaolin,WANG Jinhui,WANG Libo,PIAO Xianmei

• 1:哈尔滨医科大学药学院药理学教研室
• 2:澳诺(中国)制药有限公司
• 3:哈尔滨医科大学药学院药物化学与天然药物化学教研室

摘要(Abstract)：

目的 研究铁皮石斛(Dendrobium officinale Kimura et Migo)的化学成分。方法 铁皮石斛的体积分数70%乙醇提取物经大孔吸附树脂、硅胶、ODS、toyopearl HW-40、葡聚糖凝胶等进行分离纯化，根据光谱数据鉴定化合物的结构。结果 从铁皮石斛中分离到19个单体化合物，分别鉴定为5-羟甲基糠醛(1)、(-)-丁香脂素(2)、丁香酸(3)、对羟基苯丙酸(4)、dendrocandin U(5)、柚皮素(6)、pyrrolezanthine-6-methyl ether(7)、香草酸(8)、(+)-lyoniresinol(9)、N-反式桂皮酸酰对羟基苯乙胺(10)、icariol A_2(11)、dendronbibisline B(12)、对羟基苯丙酰酪胺(13)、pollenfuran B(14)、5-methoxy-(+)-isolariciresinol(15)、4-烯丙基-2,6-二甲氧基苯基葡萄糖苷(16)、citrusin C(17)、(+)-丁香脂素-O-β-D-吡喃葡萄糖苷(18)和1-甲基-3-苯丙基-β-D-吡喃葡萄糖苷(19)。结论 化合物7、9、11、12、14、15、19为首次从铁皮石斛中分离得到。
Objective To research the chemical constituents of Dendrobium officinale Kimura et Migo.Methods Ethanol extract with a 70%volume fraction of Dendrobium officinale Kimura et Migo was separated and purified by macroporous adsorption resin, silica gel, octadecylsilyl(ODS),toyopearl HW-40,dextran gel and so on.And the structures of the compounds were identified by spectroscopies.Results Nineteen compounds were isolated from Dendrobium officinale Kimura et Migo.They were identified as 5-hydroxymethy-furaldehyde(1),(-)-syringaresinol(2),syringic acid(3),p-hydroxy-phenylpropionic acid(4),dendrocandin U(5),naringin(6),pyrrolezanthine-6-methyl ether(7),vanillic acid(8),(+)-lyoniresinol(9),N-trans-cinnamoyl tyramine(10),icariol A_2(11),dendronbibisline B(12),p-hydroxyphenylpropionyl tyramine(13),pollenfuran B(14),5-methoxy-(+)-isolariciresinol(15),4-allyl-2,6-dimethoxyphenyl glucoside(16),citrusin C(17),(+)-syringaresinol-O-β-D-glucopyranoside(18),and 1-methyl-3-phenylpropyl-β-D-glucopyranoside(19) respectively.Conclusion Compounds 7,9,11,12,14,15 and 19 are found from Dendrobium officinale Kimura et Migo for the first time.

关键词(KeyWords)： 铁皮石斛;化学成分;分离;鉴定
Dendrobium officinale Kimura et Migo;chemical constituent;isolation;identification

Abstract:

Keywords:

基金项目(Foundation): 国家重大新药创制项目(2018ZX09735005);; 国家自然科学基金资助项目(81703760)

作者(Author): 丰明凤,高岩,白嘉璇,尤丽娟,孙晓琳,王金辉,王立波,朴贤美
FENG Mingfeng,GAO Yan,BAI Jiaxuan,YOU Lijuan,SUN Xiaolin,WANG Jinhui,WANG Libo,PIAO Xianmei

DOI: 10.14066/j.cnki.cn21-1349/r.2021.1378

参考文献(References)：

• [1] LIU J,MANG X Y.Review on pharmacological effects of Dendrobium officinale[J].Agriculture of Henan(河南农业),2019,1(3):58-59.
• [2] LI X M,WU P,XIE X J,et al.Effects of Dendrobii officinalis Caulis on serum lipid,TNF-α and IL-6 in apolipoprotein E-deficient mice [J].Chinese Journal of Experimental Traditional Medical Formulae(中国实验方剂学杂志),2013,19(18):270-274.
• [3] YAN X M,YANG J Y,LI Y,et al.Recent research situation of genuine regional drug Dendrobium officinale[J].Guangming Journal of Chinese Medicine(光明中医),2020,35(17):2783-2785.
• [4] LIN M L.Market status of Dendrobium officinale in Fujian,Guangdong and Zhejiang[J].Flower Plant & Penjing(花木盆景),2013(11):36.
• [5] CAI X J,XU H X,LIN S H,et al.Separation and identification of the new chemical constituent from rhubarb charcoal and its formational rule [J].Journal of Chinese Hospital Pharmacy(中国医院药学杂志),2018,38(22):2336-2339.
• [6] LU Q,LI H B,YAO X S,et al.Chemical constituents from rhizomes of Cimicifuga dahurica [J].Chinese Traditional and Herbal Drugs(中草药),2019,50(14):3261-3268.
• [7] LI Y.Studies on the chemical constituents of Dendrobium candidum(铁皮石斛化学成分的研究)[D].Beijing:Chinese Academy of Medical Sciences& Peking Union Medical College,2009.
• [8] LIU J,LI C J,YANG J Z et al.Chemical constituents from stems of Clausena lansium[J].Chinese Traditional and Herbal Drugs(中草药),2016,35(3):125-128.
• [9] YANG L,LIU S J,LUO H R,et al.Two new dendrocandins with neurite outgrowth-promoting activity from Dendrobium officinale[J].J Asian Nat Prod Res,2015,17(2):125-131.
• [10] LIU X Y,JI C J,PENG W W.Chemical constituents from Citrus aurantium L [J].Chinese Pharmaceutical Journal(中国药学杂志),2018,53(19):1627-1631.
• [11] FEI Y H,CHEN Z,LI X R,et al.Chemical constituents from seeds of Helianthus annuus [J].Chinese Traditional and Herbal Drugs(中草药),2014,45(5):631-634.
• [12] LI M,ZHANG Z G,SHI J Y,et al.Isolation and identification of chemical constituents from Chaenomeles sinensis (Thouin) Koehne [J].Chinese Journal of Medicinal Chemistry(中国药物化学杂志),2020,30(8):481-486.
• [13] JI M C,XIAO S J,JIANG S Y,et al.Chemical constituents from Pothos chinensis[J].Chinese Traditional and Herbal Drugs(中草药),2015,46(1):28-32.
• [14] CHENG S,MA Y Y,PENG C Y,et al.Chemical constituents from the ethyl acetate effective parts of Smilacis Glabrae Rhizoma [J].Journal of Chinese Medicinal Materials(中药材),2021,44(1):79-83.
• [15] CHENG L,GUO D L,ZHANG M S,et al.Dihydrophenanthrofurans and bisbibenzyl derivatives from the stems of Dendrobium nobile[J].Fitoterapia,2020,143:1-5.
• [16] GUO J L,YANG Y N,HE J,et al.Two new furan derivatives from bee-collected rape pollen [J].J Asian Nat Prod Res,2011,13(10):930-933.
• [17] YAO H L,GAO H,YAN S D,et al.Chemical constituents from Spiraea pubescens[J].Chinese Traditional and Herbal Drugs(中草药),2016,47(9):1486-1491.
• [18] PANG Y X,YUAN C,HU X,et al.Chemical constituents of Li Minority Herb Blumea balsamifera[J].Journal of Chinese Medicinal Materials(中药材),2019,42(1):91-95.
• [19] XIAO Y Y,GOU P,XIE H H.Phenylpropanoids from Lentopodium lenotopodioides[J].Journal of Tropical and Subtropical Botany(热带亚热带植物学报),2017,25(2):195-201.
• [20] CHEN C F,LOU S,LOU H Y.Glycoside constituents from Bletilla striata[J].Chinese Traditional and Herbal Drugs(中草药),2019,20(50):4879-4883.