李春梅,吴春华,王涛,刘二伟,陈秋,张祎
LI Chun-mei,WU Chun-hua,WANG Tao,LIU Er-wei,CHEN Qiu,ZHANG Yi(Key Laboratory of Traditional Chinese Medicinal Chemistry and Analytical Chemistry of Tianjin

• 1:天津市中药化学与分析重点实验室

摘要(Abstract)：

目的研究中药北刘寄奴(Siphonostegia chinensis Benth.)的化学成分。方法采用正相硅胶、反相ODS、Sephadex LH-20等柱色谱及高效液相色谱等手段进行分离纯化,并通过理化性质与光谱分析方法鉴定化合物的结构。结果从北刘寄奴体积分数95%乙醇提取物中分离鉴定10个单体化合物,分别为反式对羟基肉桂酸((E)-p-hydroxycinnamic acid,1)、咖啡酸(caffeic acid,2)、syring-in(3)、(7S,8R)-dehydrodiconiferyl alcohol 9'-β-glucopyranoside(4)、vanillyl alcohol-4-O-β-D-gluco-pyranoside(5)、2,6-二甲氧基-4-羟基苯酚-1-O-葡萄糖苷(2,6-dimethoxy-4-hydroxyphenol 1-O-glu-copyranoside,6)、3,5-dimethoxy-4-hydroxybenzyl alcohol 4-O-β-D-glucopyranoside(7)、rubinaphthinA(8)、rel-5-(3S,8S-dihydroxy-1R,5S-dimethyl-7-oxa-6-oxobicyclo[3,2,1]-oct-8-yl)-3-methyl-2Z,4E-pentadienoic acid(9)、(6S,9R)-roseoside(10)。结论化合物2～10为首次从阴行草属植物中分离得到。
Objective To study the chemical constituents from the whole plant of Siphonostegia chinensis Benth..Methods The compounds were isolated by chromatographic methods such as silica gel,ODS,Sephadex LH-20 and HPLC,and identified by the chemical and physical methods,especially spectral analysis.Results Ten compounds were isolated and identified as(E)-p-hydroxycinnamic acid(1),caffeic acid(2),syringin(3),(7S,8R)-dehydrodiconiferyl alcohol 9′-β-glucopyranoside(4),vanillyl alcohol-4-O-β-D-glucopyranoside(5),2,6-dimethoxy-4-hydroxyphenol 1-O-glucopyranoside(6),3,5-dimethoxy-4-hydroxybenzyl alcohol 4-O-β-D-glucopyranoside(7),rubinaphthin A(8),rel-5-(3S,8S-dihydroxy-1R,5S-dimethyl-7-oxa-6-oxobicyclo-oct-8-yl)-3-methyl-2Z,4E-pentadienoic acid(9)and(6S,9R)-roseoside(10).Conclusions Compounds 2-10 are isolated from Siphonostergia genus for the first time.

关键词(KeyWords)： 阴行草属;北刘寄奴;化学成分;结构鉴定
Siphonostergia genus;Siphonostegia chinensis Benth.;chemical constituent;structure identification

Abstract:

Keywords:

基金项目(Foundation): 天津市高等学校科技发展基金计划项目(20090201);; 国家科技重大专项项目-重大新药创制(2011ZX09307-002-01,2012ZX09103201-031);; 教育部留学回国人员科研启动基金资助项目(教外司留[2010]609号)

作者(Author): 李春梅,吴春华,王涛,刘二伟,陈秋,张祎
LI Chun-mei,WU Chun-hua,WANG Tao,LIU Er-wei,CHEN Qiu,ZHANG Yi(Key Laboratory of Traditional Chinese Medicinal Chemistry and Analytical Chemistry of Tianjin

DOI: 10.14066/j.cnki.cn21-1349/r.2012.05.003

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