RP-HPLC法测定清胰利胆颗粒中绿原酸和芍药苷的含量Determination of chlorogenic acid and paeoniflorin in Qingyilidan granules by RP-HPLC
冯川,刘唯芬,慕善学,张艳君,赵怀清
FENG Chuan1,LIU Wei-fen1,MU Shan-xue1,ZHANG Yan-jun1,ZHAO Huai-qing2(1.Vocational and Technology School
摘要(Abstract)：
目的建立同时测定清胰利胆颗粒中绿原酸和芍药苷含量的方法。方法采用HPLC法。色谱柱:Kromasil C18柱(250 mm×4.6 mm,5μm),流动相:乙腈(A)-体积分数为0.1%的磷酸水溶液(B),梯度洗脱程序:0 min-10%(φ)A、9 min-13%(φ)A、13 min-13%(φ)A、14 min-16%(φ)A、22min-17%(φ)A、30 min-17%(φ)A、40 min,40%(φ)A,流速:1.0 mL.min-1,检测波长:327 nm(0～14 min)和230 nm(14～40 min)。结果绿原酸和芍药苷质量浓度分别在5.2～51.6 mg.L-1(r=0.999 6,n=6)、9.9～99.2 mg.L-1(r=0.999 8,n=6)内与峰面积线性关系良好,平均回收率分别为100.5%(RSD=1.9%,n=6)、100.2%(RSD=2.2%,n=6)。结论该方法可作为清胰利胆颗粒质量控制方法之一。
Objective To develop an HPLC method for simultaneous determination of chlorogenic acid and paeoniflorin in Qingyilidan granules(traditional Chinese medicines).Methods Analyses were carried out on Kromasil C18 column(250 mm×4.6 mm,5 μm).The mobile phase was acetonitril(A)-1%(φ) acetic acid(B)with gradient elution(0 min,10%(φ)A;9 min,13%(φ)A;13 min,13%(φ)A;14 min,16%(φ)A;22 min,17%(φ)A;30 min,17%(φ)A;40 min,40%(φ)A and the flow rate was 1.0 mL · min-1,the detection wavelength was set at 327 nm(0-14 min)and 230 nm(14-40 min).Results The linear calibration curve were obtained in the concentration range of 0.676-10.14 mg · L-1(r=0.999 6,n=5) for chlorogenic acid and 0.276-3.680(r=0.999 8,n=5) for paeoniflorin.The mean recoveries of chlorogenic acid and paeoniflorin were 96.7%(RSD=1.7%,n=6) and 96.4%(RSD=1.0%,n=6),respectively.Conclusions The method can be used to contro1 the quality of Qingyilidan granules,which provides the advantage of quickness,simplicity and repeatability.
关键词(KeyWords)：
HPLC;清胰利胆颗粒;绿原酸;芍药苷
HPLC;Qingyilidan granules;chlorogenic acid;paeoniflorin
基金项目(Foundation):
作者(Author):
冯川,刘唯芬,慕善学,张艳君,赵怀清
FENG Chuan1,LIU Wei-fen1,MU Shan-xue1,ZHANG Yan-jun1,ZHAO Huai-qing2(1.Vocational and Technology School
DOI: 10.14066/j.cnki.cn21-1349/r.2012.03.008
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