袭荣刚,吴立军,王立波,王晓波,高慧媛,康廷国
XI Rong-gang,WU Li-jun,WANG Li-bo,WANG Xiao-bo,GAO Hui-yuan,KANG Ting-guo

• 1:辽宁中医药大学药学院
• 2:中国人民解放军第210医院
• 3:沈阳药科大学基于靶点的药物设计与研究教育部重点实验室

摘要(Abstract)：

目的以延胡索乙素为指标成分对中药延胡索的提取工艺进行优化,以利于工业化生产。方法采用响应曲面法对提取工艺进行优化,通过考察乙醇体积分数、提取时间、溶媒用量及其交互作用对中药延胡索中延胡索乙素提取率的影响。模拟得出了延胡索乙素的提取回归方程,确定了延胡索乙素的最佳提取条件。结果优化得到的最佳工艺参数:乙醇体积分数为87.16%,提取时间为105.13 min,液料比为1∶7.13。在该工艺条件下,提取物中延胡索乙素的含量质量分数为0.183%。结论该提取工艺对延胡索提取物中的延胡索乙素提取优化效果显著,方法稳定、可行。
Objective To optimize the extraction process condition of tetrahydropalmatine from Corydalis yanhusuo W.T.Wang.Methods By using response surface methodology(RSM),three factors,including ethanol volumetric concentrations,liquid to solid ratio and extraction time were optimized.The content of tetrahydropalmatine in samples extracted under the optimum condition was determined by the HPLC analysis method.Results The optimum condition was as follow: 87.16% of ethanol concentration; 105.13 min of extraction duration and the ratio of material: 1: 7.13.Extracted under the optimal processing condition,the tetrahydropalmatine content was 0.183%.Conclusions The optimal condition of the extraction of tetrahydropalmatine form Corydalis yanhusuo process was efficient,stable and feasible.

关键词(KeyWords)： 延胡索;延胡索乙素;响应曲面
Corydalis yanhusuo W.T.Wang;tetrahydropalmatine;response surface methodology

Abstract:

Keywords:

基金项目(Foundation): 国家十一五重大新药创制项目(2009ZXJ09002-019)

作者(Author): 袭荣刚,吴立军,王立波,王晓波,高慧媛,康廷国
XI Rong-gang,WU Li-jun,WANG Li-bo,WANG Xiao-bo,GAO Hui-yuan,KANG Ting-guo

DOI: 10.14066/j.cnki.cn21-1349/r.2017.01.015

参考文献(References)：

• [1]国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015:139-140.
• [2]王红,田明,王淼,等.延胡索现代药理及临床研究进展[J].中医药学报,2010,38(6):108-111.
• [3]贺凯,高建莉,赵光树.延胡索化学成分,药理作用及质量控制研究进展[J].中草药,2007,38(12):1909-1912.
• [4]李光,李学兰,孙慧峰,等.响应曲面法优化铁皮石斛多糖提取条件[J].中国现代中药,2011,13(9):29-33.
• [5]陈洪燕,万明,孙金霞.均匀设计延胡索提取工艺的研究[J].湖北中医杂志,2006,28(7):54-55.
• [6]郑妮,张生潭,汪铁山,等.水蒲桃种子总多酚提取工艺的响应曲面法优化[J].中国实验方剂学杂志,2011,17(17):41-44.
• [7]张百霞,郭庆梅,王真真,等.响应曲面法优化金银花总酚酸提取工艺[J].中成药,2013,35(10):2145-2148.