张美,李维维,朱华凤,彭缨
ZHANG Mei,LI Wei-wei,ZHU Hua-feng,PENG Ying* (School of Pharmacy

• 1:沈阳药科大学药学院

摘要(Abstract)：

目的建立GC-FID含量测定方法,同时测定保妇康栓中冰片、呋喃二烯、莪术醇和莪术二酮的含量。方法采用HP-5石英毛细管色谱柱(30 m×0.25 mm,0.25μm)程序升温,流速为2.5 mL.min-1,分流比为10∶1,进样量为1μL。结果冰片、呋喃二烯、莪术醇和莪术二酮质量浓度分别在28.0～224.0(r=0.999 8)、16.0～128.0(r=0.999 9)、2.8～22.4(r=0.999 8)、4.0～32.0 mg.L-1(r=0.999 9)内线性关系良好,平均回收率分别为103.7%、91.6%、102.5%、99.5%,RSD分别为4.7%、3.6%、3.9%、4.3%(n=9)。结论本方法可为保妇康栓的质量控制提供科学依据。
Objective To establish a GC-FID method of Baofukang Suppository and develop this method for determination of borneol,furanodiene,curcumol and curdione at the same time.Methods A HP-5 quartz capillary column(30 m×0.25 mm,0.25 μm) programmed temperature was used at the flow rate of 2.5 mL · min-1 with split ratio of 10∶ 1;injection volume was 1 μL.Results Borneol,furanodiene,curcumol and curdione were linear in the range of 28.0-224.0(r=0.999 8),16.0-128.0(r=0.999 9),2.8-22.4(r=0.999 8),4.0-32.0 mg · L-1(r=0.999 9),the average recoveries were 103.7%,91.6%,102.5%,99.5%,RSD were 4.7%,3.6%,3.9%,4.3%(n=9),respectively.Conclusions The validated method is simple and shows good accuracy and repeatability,which can provide scientific evidence for the quality control of Baofukang Suppository.

关键词(KeyWords)： 毛细管气相色谱法;保妇康栓;冰片;呋喃二烯;莪术醇;莪术二酮;含量测定
capillary GC analysis;Baofukang suppository;borneol;furanodiene;curcumol;curdione;content determination

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 张美,李维维,朱华凤,彭缨
ZHANG Mei,LI Wei-wei,ZHU Hua-feng,PENG Ying* (School of Pharmacy

DOI: 10.14066/j.cnki.cn21-1349/r.2013.08.006

参考文献(References)：

• [1]国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2010:930.
• [2]李国栋,许付,沈爱军.莪术油的研究进展[J].中国药学杂志,2002,37(11):806-809.
• [3]张秋琼,刘文志,张周菊,等.GC法测定复方苦参栓剂中冰片的含量[J].湖北中医学院学报,2010,12(5):36-37.
• [4]陈瑾,余继英.气相色谱-质谱联用法测定复方蒲公英栓中冰片的含量[J].中国实用医药,2011,6(36):7-8.
• [5]伊文敏,汪秀月.气相色谱法测定消糜栓中冰片(龙脑)的含量[J].安徽医药,2010,14(4):415-416.
• [6]YANG F Q,LI S P,ZHAO J,et al.Optimization ofGC-MS conditions based on resolution and stability ofanalytes for simultaneous determination of nine ses-quiterpenoids in three species of Curcuma rhizomes[J].Journal of Pharmaceutical and Biomedical Analy-sis,2007,43:73-82.
• [7]YANG F Q,LI S P,CHEN Y,et al.Identification andquantitation of eleven sesquiterpenes in three speciesof Curcuma rhizomes by pressurized liquid extractionand gas chromatography-mass spectrometry[J].Jour-nal of Pharmaceutical and Biomedical Analysis,2005,39:552-558.
• [8]李想,孙艳涛,张振秋,等.毛细管气相色谱法测定莪术油中β-榄香烯、莪术醇、吉马酮和莪术二酮的含[J].药物分析杂志,2009,29(11):1832-1836.
• [9]杨丰庆,李绍平,陈莹,等.不同来源获术中获术醇、吉马酮、获术二酮GC-MS定量分析[J].药物分析杂志2005,25(7):827-829.
• [10]刘姝畅,孙艳涛,李想,等.GC测定莪术残油中莪术醇、吉马酮和莪术二酮的含量[J].中成药,2010,32(9):1634-1635.
• [11]王玉生,汪小根,唐铁鑫.保妇康栓中莪术醇的毛细管气相色谱法含量测定[J].中成药,2004,26(6):466-467.
• [12]杨日丽,刘慧,任宏.反相高效液相法测定保妇康栓中莪术醇的含量[J].南通大学学报(医学版),2007,27(3):187-188.
• [13]周志林,李芸芳.气相色谱法测定麝香痔疮栓中冰片的含量[J].中南民族大学学报(自然科学版),2008,27(4):47-49.
• [14]刘文,潘健,陈文秋.麝香痔疮栓中樟脑的定量分析[J].中国民族民间医药,2012,(2):28-29.
• [15]张翠英,王青晓,李振国.毛细管气相色谱法测定前列泌尿栓中冰片的含量[J].中成药,2007,29(4):601-603.