曹阳阳,郭斌
CAO Yang-yang1,GUO Bin2(1.Graduate School

• 1:辽宁医学院研究生院
• 2:辽宁医学院附属第一医院

摘要(Abstract)：

目的考察Ⅱ型ZTC1+1天然澄清剂在萝摩多糖提取中的提纯效果。方法以硫酸-苯酚法测定含量,通过正交实验进行优化,确定理想的Ⅱ型ZTC1+1天然澄清剂处理工艺。采用Ⅱ型ZTC1+1天然澄清剂对提取出的萝摩多糖复合物进行纯化得萝摩粗多糖。结果Ⅱ型ZTC1+1天然澄清剂能有效提高萝摩多糖的提取量,并与澄清剂2组份的比例,反应温度有关。结论Ⅱ型ZTC1+l天然澄清剂纯化萝摩总糖的最佳工艺为A3B1C2D1,即A、B组分的体积分数分别为1%、2%,药液的浓缩程度为m(药物/g):V(水/mL)=1:10,温度为70℃,加热时间为B组分加入后加热1 h,A组分加入后加热1 h。
Objective To study the effect of type ⅡZTC1+1 natural clarifying agent used in the purification of Japanese metaplexis polysaccharide.Methods The content of Japanese metaplexis polysaccharide was determined by phenol-sulphuric acid.The method was optimized by orthogonal experiment.Type Ⅱ ZTC1 + 1 natural clarifying agent was used to refine the extracted Japanese metaplexis polysaccharide compound to get crude polysaccharide.Results The result showed that type ⅡZTC1+1 natural clarifying agent could effectively improve the content of Japanese metaplexis polysaccharide,the effect was relates to the proportion of two components and temperature.Conclusions The optimal condition of Type ⅡZTC1+1 natural clarifying agent used in Japanese metaplexis polysaccharide is A1B3C1D2.

关键词(KeyWords)： 天然澄清剂;萝摩多糖;纯化
natural clarifying agent;Japanese metaplexis polysaccharide;purification

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 曹阳阳,郭斌
CAO Yang-yang1,GUO Bin2(1.Graduate School

DOI: 10.14066/j.cnki.cn21-1349/r.2012.12.009

参考文献(References)：

• [1]梁.陶弘景.本草经集注辑校本[M].北京:北京人民卫生出版社,1994:298.
• [2]史德芳,高虹,周明,等.II型ZTC1+1天然澄清剂在巴西菇多糖提纯中的应用[J].湖北农业科学,2011,50(6):1251-1254.
• [3]林江博,李淑芬,杨鸿玲,等.ZTC1+1天然澄清剂在桑叶多糖提纯中的应用[J].化学工业与工程,2008,25(1):40-43.
• [4]龚慕辛,贾春伶.天然澄清剂在中药提取液精制中的应用[J].北京中医,2001,6:43-43.
• [5]郭朝晖,梁卿,刘霞,等.Ⅱ型ZTC1+1天然澄清剂在黄芪多糖提纯中的应用[J].时珍国医国药,2005,16(9):876-877.
• [6]高静,刘培勋,龙伟.Ⅱ型ZTC1+1天然澄清剂精制仙鹤复方总糖的研究[J].中国新药杂志,2008,17(15):1335-1338.
• [7]史德芳,高虹,周明,等.II型ZTC1+1天然澄清剂在巴西菇多糖提纯中的应用[J].湖北农业科学,2011,50(6):1251-1254.
• [8]高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006.
• [9]付焱,郭毅,张嫡群,等.欧亚旋覆花中多糖的苯酚-硫酸法测定[J].中草药,2006,37(4):544-546.