RP-HPLC法测定葫芦素B月桂酸酯纳米乳剂中药物的含量Determination of cucurbitacin B laurate in nanoemulsions by RP-HPLC
李乐,邓意辉,石莉,董晓辉,卢懿,洪维为
LI Le,DENG Yi-hui,SHI Li,DONG Xiao-hui,LU Yi,HONG Wei-wei(School of Pharmacy
摘要(Abstract)：
目的建立测定葫芦素B月桂酸酯纳米乳剂中药物含量的方法。方法采用Diamonsil ODS柱(钻石)C18柱(200 mm×4.6 mm,5μm),流动相为甲醇-水(体积比为95∶5),流速1.0 mL.min-1,检测波长228 nm,柱温为室温。结果在本试验条件下,葫芦素B月桂酸酯与辅料及有关物质分离度均符合要求,其质量浓度在15~150 mg.L-1内与峰面积呈良好线性关系(r=0.999 9,n=5),方法平均回收率为99.98%,RSD为0.1%(n=9)。结论该方法简单、快捷,可用于葫芦素B月桂酸酯纳米乳剂中药物含量的测定。
Objective To establish an RP-HPLC method for the determination of cucurbitacin B laurate in nanoemulsions.Methods The separation was performed with a Diamonsil ODS column(200 mm×4.6 mm,5 μm) at room temperature,and the mobile phase was consisted of methanol-water(V∶V=95∶5),the flow rate was 1.0 mL·min-1.Cucurbitacin B laurate was detected at 228 nm.Results There was a good linear relationship between A and concentration over the range of 15-150 mg·L-1(r=0.999 9,n=5).The average recovery was 99.98%.The RSD was 0.1%(n=9).Conclusions This method is applicable for the determination of cucurbitacin B laurate in nanoemulsions.
关键词(KeyWords)：
反相高效液相色谱法;葫芦素B月桂酸酯;纳米乳剂;含量测定
RP-HPLC;cucurbitacin B laurate;nanoemulsion;content determination
基金项目(Foundation):
作者(Author):
李乐,邓意辉,石莉,董晓辉,卢懿,洪维为
LI Le,DENG Yi-hui,SHI Li,DONG Xiao-hui,LU Yi,HONG Wei-wei(School of Pharmacy
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