张锦红,王维宁,刘瑀曦,王延年,裴月湖,刘晓秋
ZHANG Jin-hong1,WANG Wei-ning2,LIU Yu-xi1,WANG Yan-nian1,PEI Yue-hu1,LIU Xiao-qiu1(1.School of Traditional Chinese Materia Medica

• 1:沈阳药科大学中药学院
• 2:辽宁省食品药品检验所

摘要(Abstract)：

目的考察盐炙对关黄柏水煎液中主要生物碱类及柠檬苦素类成分溶出的影响。方法采用HPLC法比较用分析纯NaCl、MgCl2、KCl和食盐炮制的关黄柏样品中3种主要生物碱小檗碱、药根碱、巴马汀的峰面积。色谱柱:Diamonsil ODS C18柱(250 mm×4.6 mm,5μm),流动相:乙腈(A)-体积分数为0.1%的磷酸溶液(B)(梯度洗脱程序:0～20 min:90%B～65%B,20～35 min:65%B～50%B),流速:0.8 mL.min-1,检测波长:345 nm。同时采用分光光度法测定水煎后药渣中柠檬苦素类成分的含量。结果不同种类的盐炮制的关黄柏中生物碱类成分的峰面积均有变化。药渣中的柠檬苦素成分含量也均有变化。结论盐炙对关黄柏中主要成分的水中溶出有较大影响,为阐明盐炙的炮制机理提供一定的依据。
Objective To investigate the influence of salt-prepared on the dissolution of alkaloids and limonins in Phellodendri Amurensis Cortex.Methods HPLC was used to compare the fingering peak areas of 3 kinds of alkaloids in processed Phellodendri Amurensis Cortex with different kinds of salt,such as NaCl,MgCl2,KCl and so on.The separation was performed on Diamonsil ODS-C18 column.(250 mm×4.6 mm,5 μm).The mobile phase was composed of acetonitrile(A)and 0.1% phosphoric acid in water(B)using the following gradient program:0-20 min:90%-65% B,20-35 min:65%-50% B.Detection wavelength was set at 345 nm with a flow rate of 0.8 mL·min-1.The contents of limonoids in gruffs after water-extract were determined by spectrophotometry. Results The peak areas of alkaloids were all changed after processed with different salt.The contents of limonoids in gruffs were also changed. Conclusions Salt-prepared has great influence on the dissolution of compositions in Phelodendri amurensis Cortex,and it can provide a basis for clarifying the mechanism of salt-prepared.

关键词(KeyWords)： 关黄柏;盐炙;生物碱;柠檬苦素;溶出
Phellodendri Amurensis Cortex;salt-prepared;alkaloid;limonoid;dissolution

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 张锦红,王维宁,刘瑀曦,王延年,裴月湖,刘晓秋
ZHANG Jin-hong1,WANG Wei-ning2,LIU Yu-xi1,WANG Yan-nian1,PEI Yue-hu1,LIU Xiao-qiu1(1.School of Traditional Chinese Materia Medica

DOI: 10.14066/j.cnki.cn21-1349/r.2012.07.006

参考文献(References)：

• [1]国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药出版社,2010:137.
• [2]龚千锋.中药炮制学:第2版[M].北京:中国中医药出版社,2007:208-209.
• [3]陈发奎,刘晓秋.中药有效成分含量测定[M].北京:人民卫生出版社,2008:1858.
• [4]YOUSHITERU I,SATH Y,MASUMI O.Phenolic con-stituents of Phellodendron Amurense bark[J].Phyto-chemistry,1994,35(1):209-215.
• [5]廉莲.黄柏的炮制原理研究[D].沈阳:辽宁中医药大学,2008:5.
• [6]战旗,张学兰,王苓,等.黄柏及其炮制品水提液的成分比较[J].中成药,1999,21(3):126-128.