米文珍,张国刚,陈丽娟,潘春媛,王庆,贺杰
MI Wen-zhen,ZHANG Guo-gang,CHEN Li-juan,PAN Chun-yuan,WANG Qing,HE Jie(School of Traditional Chinese Materia Medica

• 1:沈阳药科大学中药学院基于靶点的药物设计与研究教育部重点实验室

摘要(Abstract)：

目的建立紫花苜蓿中考迈斯托醇的含量测定方法。方法采用RP-HPLC法,使用Kromasil C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-体积分数为0.04%磷酸溶液(体积比为60∶40),流速为1.0 mL.min-1,检测波长为345 nm,柱温为25℃。结果考迈斯托醇质量浓度在0.75～22.50mg.L-1内呈良好的线性关系(r=0.999 6,n=5),平均回收率为98.45%,RSD为2.2%(n=6)。结论RP-HPLC法适用于紫花苜蓿中考迈斯托醇含量的测定。
Objective To develop an HPLC method for the determination of coumestrol in Medicago sativa L..Methods A Kromasil C18 column(250 mm×4.6 mm,5 μm)with a solvent system consisting of MeOH-H2O[w(H3PO4)=0.04%](V∶V=60∶40)was used with a flow rate of 1.0 mL·min-1 and the detecting wavelength was set at 345 nm.The column temperature was 25 ℃.Results The coumestrol curve showed good linear correlation within 0.75-22.50 mg·L-1(r=0.999 6).The average recovery of coumestrol was 98.45% with RSD of 2.2%(n=6).Conclusions The method is simple and can be used to determine coumestrol with satisfactory accuracy and reproducibility.

关键词(KeyWords)： 紫花苜蓿;考迈斯托醇;反相高效液相色谱法;含量测定
Medicago sativa L.;coumestrol;RP-HPLC;content determination

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 米文珍,张国刚,陈丽娟,潘春媛,王庆,贺杰
MI Wen-zhen,ZHANG Guo-gang,CHEN Li-juan,PAN Chun-yuan,WANG Qing,HE Jie(School of Traditional Chinese Materia Medica

DOI: 10.14066/j.cnki.cn21-1349/r.2010.04.002

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