黄肖霄,李殿明,李玲芝,郭东东,任瑞涛,宋少江
HUANG Xiao-xiao1,LI Dian-ming2,LI Ling-zhi1,GUO Dong-dong1,REN Rui-tao2,SONG Shao-jiang1(1.Key Laboratory of Structure-Based Drug Design & Discovery of Ministry of Education

• 1:沈阳药科大学基于靶点的创新药物研究与设计重点实验室
• 2:哈药集团中药二厂

摘要(Abstract)：

目的对蔷薇科山楂属植物山楂(Crataegus pinnatifida Bge.)叶的化学成分进行研究。方法运用硅胶柱色谱﹑Sephadex LH-20柱色谱﹑ODS柱色谱、制备HPLC等分离手段进行化学成分的分离纯化,根据理化性质及波谱数据鉴定其结构。结果从山楂叶体积分数80%乙醇水溶液的提取物中分离得到7个化合物,分别鉴定为α-tetrahydrobisabolen-2,5,6-triol(1)、10,11-dihydroxynero-lidol(2)、3,5,4'-三甲氧基-4-羟基-联苯(3,5,4'-trimethoxy-4-hydroxyl-biphenyl,3)、(+)-7R,8S-5-methoxy dihydrodehydroconiferyl alcohol(4)、5,4'-二甲氧基-联苯-4-羟基-3-O-β-D-葡萄糖苷(5,4'-dimethoxy-biphenyl-4-ol-3-O-β-D-glucoside,5)、槲皮素(quercetin,6)、牡荆素(vitexin,7)。结论化合物1为新天然产物,化合物2～4为首次从山楂属植物中分离得到。
Objective To study the chemical constituents of the leaves of Crataegus pinnatifida Bge..Methods Seven compounds were isolated by chromatography on silica gel column,Sephadex LH-20,ODS chromatography column and HPLC,their structures were confirmed on the basis of physico-chemical constants and spectroscopic analysis.Results Seven compounds were isolated from 80% ethanol-water extraction and their structures were identified as α-tetrahydrobisabolen-2,5,6-triol(1),10,11-dihydroxynerolidol(2),3,5,4′-trimethoxy-4-hydroxyl-biphenyl(3),(+)-7R,8S-5-methoxy dihydrodehydroconiferyl alcohol(4),5,4′-dimethoxy-biphenyl-4-ol-3-O-β-D-glucoside(5),quercetin(6),vitexin(7).Conclusions Compound 1 is a new natural product and compounds 2-4 are isolated from Crataegus genus for the first time.

关键词(KeyWords)： 山楂叶;化学成分;结构鉴定
Crataegus pinnatifida Bge.leaf;chemical constituent;structure identification

Abstract:

Keywords:

基金项目(Foundation): 国家基础科学人才培养基金——能力提高(科研训练)资助项目(J1103606)

作者(Author): 黄肖霄,李殿明,李玲芝,郭东东,任瑞涛,宋少江
HUANG Xiao-xiao1,LI Dian-ming2,LI Ling-zhi1,GUO Dong-dong1,REN Rui-tao2,SONG Shao-jiang1(1.Key Laboratory of Structure-Based Drug Design & Discovery of Ministry of Education

DOI: 10.14066/j.cnki.cn21-1349/r.2012.05.005

参考文献(References)：

• [1]陈佳,宋少江.山楂的研究进展[J].中药研究与信息,2005,7(7):20-23.
• [2]MIYAZAWA M,FUNATSU Y,KAMEOKA H,et al.Biotransformation of(-)-alpha-bisabolol to(-)-al-pha-tetrahydrobisabolen-2,5,6-triol by Aspergillus ni-ger[J].Chemistry Express,1992,7(3):217-220.
• [3]DIAZ J G,GOEDKEN V L,HERZ W.Sesquiterpene lactones and other constituents of Mikania rimachii and Mikania microptera[J].Phytochemistry,1992,31(2):597-608.
• [4]CARDONA M L,FERNANDEZ I,PEDRO J R,et al.Xanthones from Hypericum reflexum[J].Phytochemis-try,1990,29(9):3003-3006.
• [5]CHIN Y W,CHAI H B,KELLER A D,et al.Lignans and other constituents of the fruits of Euterpe oleracea(Ac3ai)with antioxidant and cytoprotective activities[J].J Agric Food Chem,2008,56:7759-7764.
• [6]LI L Y,SEERAM N P.Maple syrup phytochemicals include lignans,coumarins,a stilbene,and other previ-ously unreported antioxidant phenolic compounds[J].J Agric Food Chem,2010,58:11673-11679.
• [7]陈佳,宋少江,赫军,等.山楂叶中的一个新联苯苷[J].沈阳药科大学学报,2006,7(7):430-431.
• [8]张培成,徐绥绪.山楂叶化学成分研究[J].药学学报,2001,36(10):754-757.