李渤,王乃利,姚新生,胡江春,王书锦
LI Bo 1; WANG Nai-li 1; YAO Xin-sheng 1; HU Jiang-chun 2; WANG Shu-jin 2 (1. School of Traditional Chinese Materia Medica;

• 1:沈阳药科大学中药学院
• 2:沈阳药科大学中药学院
• 3:沈阳药科大学中药学院
• 4:中国科学院应用生态研究所微生物研究室

摘要(Abstract)：

目的从海洋微生物的次级代谢产物中发现新的具有抗肿瘤活性的新化合物以用于新的抗肿瘤药物的研制开发。方法以稻瘟霉孢子形态变异为指标的生物活性物质筛选模型 ,对 2 0 0株海洋微生物资源菌株进行了反复筛选。结果得到了 6株在其发酵液中能够产生较强生物活性物质的菌株 ,即海豚葡萄球菌 (Staphylococusdelphini)、拟青霉 (Paecilomycessp )、土曲霉 (Aspergillusterreus)、青霉 (Penicilliumsp .)、中华拟青霉 (Sinopaecilomyces)、海洋芽孢杆菌 (Bacillusmarinus) ;并且对这 6株菌株的生长发育稳定性 ,遗传稳定性和生物活性物质的热稳定性进行了考察 ,发现其中的 4株菌株 ,即拟青霉 (Paecilomycessp )、青霉 (Penicilliumsp )、中华拟青霉 (Sinopae cilomyces)、海洋芽孢杆菌 (Bacillusmarinus)的各项稳定性良好。 结论为具有抗肿瘤活性物质的追踪分离奠定了基础
Objective To obtain new compounds with the antitumour activity from a secondary metabolite of marine microorganisms. Methods 200 strains of marine microorganisms which were isolated from the Yellow Sea, the Bohai Sea and the offing of Liaoning Province of China have been screened by the model of inducing morphological abnormalities of Pyricularia oryzae P-2b. Results 6 strains of microorganisms with a better bioactive substance in their fermented broth were obtained (Staphylococus delphini, Paecilomyces sp., Aspergillus terreus, Penicillium sp., Sinopaecilomyces, Bacillus marinus);Four of the strains Paecilomyces sp., Penicillium sp., Sinopaecilomyces, Bacillus marinus exhibited better stability of inheritance and growth. Conclusions Their bioactive substances had better stability of heating. All of the above results will be helpful for the separation of antitumour bioactive compounds.

关键词(KeyWords)： 海洋微生物;稻瘟霉;抗肿瘤活性
marine microorganisms; Pyricularia oryzae; antitumour activity

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 李渤,王乃利,姚新生,胡江春,王书锦
LI Bo 1; WANG Nai-li 1; YAO Xin-sheng 1; HU Jiang-chun 2; WANG Shu-jin 2 (1. School of Traditional Chinese Materia Medica;

参考文献(References)：

• [1]王书锦,胡江春,薛德林,等.中国黄海、渤海、辽宁近海地区海洋微生物资源的研究[J].锦州师范学院学报,2001,22(1):1-5.
• [2]KobayashiH ,NamikoshiM ,YoshimotoT ,etal.Ascreeningmethodforantimitoticandantifungalsub stancesusingconidiaofPyriculariaoryzae,modificationandaapplicationtotropicalmarinefungi[J].JAntibi otics,1996,49(9):873-879.
• [3]KobayashiH ,SunagaG ,FurihataK ,etal.IsoiationandstructureofanantifungalantibioticfusarielinA ,andrelatedcompoundsproducedbyaFusariumsp.[J].JAntibiotics,1995,48:42-52.
• [4]SekitaS ,YoshihiraK ,NatoriS ,etal.Chaetoglobo sins,cytotoxic10(Indol3 yl) cyto charasansfromChaetomiumsp.[J].ChemPharmBull,1982,30:1609-1617.
• [5]HuK ,DongAJ,YaoXS,etal.Antineoplastica gents;I.ThreespirostanolglycosidefromrhizomesofDioscoreacollettiivar.Hypoglauca[J].PlantaMed,1996,62:573-575.
• [6]HuK ,DongAJ,YaoXS,etal.Antineoplastica gents;II.FourfurostanolglycosidefromrhizomesofDioscoreacollettiivar.Hypoglauca[J].PlantaMed,1997,63:161-165.