范雪梅,陈刚,郭丽娜,卢轩,苏姗姗,裴月湖
FAN Xue-mei,CHEN Gang,GUO Li-na,LU Xuan,SU Shan-shan,PEI Yue-hu (School of Traditional Chinese Materia Medica

• 1:沈阳药科大学中药学院

摘要(Abstract)：

目的研究葫芦科栝楼属植物栝楼果实体积分数为90%乙醇提取物的正丁醇部分,对其中的化学成分进行分离鉴定。方法利用硅胶柱色谱,凝胶柱色谱(Sephadex LH-20),反相中低压柱色谱,制备高效液相色谱(PRE-HPLC)等手段进行分离纯化,根据理化性质和光谱数据对化合物进行了结构鉴定。结果从瓜蒌的正丁醇萃取部分分离得到10个化合物,分别鉴定为:甲基-β-D-吡喃果糖苷(methyl-β-D-frucopyranoside,1)、乙基-β-D-吡喃果糖苷(ethyl-β-D-frucopyranoside,2)、正丁基-β-D-吡喃果糖苷(n-butyl-β-D-fructopyranoside,3)、乙基-β-D-呋喃葡萄糖苷(ethyl-β-D-glu-cofuranoside4,)、正丁基-α-D-呋喃果糖苷(n-butyl-α-D-fructofuranoside5,)、正丁基-β-D-呋喃果糖苷(n-butyl-β-D-fructofuranoside,6)、bluemenol A(7)、cucumegastigmanesⅠ(8)、5-羟甲基糠醛(5-hydroxymethylfurfural9,)5、5,'-双氧甲基呋喃醛(5,5'-oxybis(methylene)difurfural1,0)。结论化合物1-8均为首次从该植物中分离得到。
Objective To isolate and identify the chemical constituents of the butanol extract part of the Trichosanthes kirilowii Maxim.ethanol extract. Methods The chemical constituents of T kirilowii Maxim.were studied by multiple chromatographic techniques(such as silica gel chromatography,Sephadex LH-20,and HPLC etc.).These compounds were identified by analysis of the spectral data and chemical properties. Results On the basis of physico-chemical evidence and spectral analysis,the structures of the 10 compounds were identified as follow:methylmethyl-β-D-frucopyranoside( 1),ethylethyl-β-D-frucopyranoside( 2),n-butylbutyl-β-D-fructopyranoside( 3),ethyl-β-D-glucofuranoside( 4),n-butylbutyl-α-D-fructofuranoside( 5),n-Butylbutyl-β-D-fructofuranoside( 6),bluemenol A( 7),cucumegastigmanesⅠ( 8),5-hydroxymethylfurfural( 9),5,5′-oxybis(methylene)difurfural( 10). Conclusions Among yielded compounds,compounds 18 were isolated from this genus for the first time.

关键词(KeyWords)： 瓜蒌;正丁醇层;结构鉴定
Trichosanthes kirilowii Maxim.;butanol extract;structure identification

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(30873368)

作者(Author): 范雪梅,陈刚,郭丽娜,卢轩,苏姗姗,裴月湖
FAN Xue-mei,CHEN Gang,GUO Li-na,LU Xuan,SU Shan-shan,PEI Yue-hu (School of Traditional Chinese Materia Medica

DOI: 10.14066/j.cnki.cn21-1349/r.2011.11.003

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