冀召帅,韩佳容,邹梅娟,聂鑫,王雪,程刚
JI Zhao-shuai,HAN Jia-rong,ZOU Mei-juan,NIE Xin,WANG Xue,CHENG Gang(School of Pharmacy

• 1:沈阳药科大学药学院

摘要(Abstract)：

目的建立测定大鼠血浆中山奈酚质量浓度的HPLC法,并用于山奈酚经灌胃给药后在大鼠体内药动学研究。方法大鼠灌胃给予200 mg.kg-1山奈酚混悬液,于给药后不同时间采集血样,以非那西丁为内标,VC为抗氧剂,采用2 mol.L-1盐酸,80℃水浴水解30 min后用乙醚萃取的方法处理血浆样品。采用RP-HPLC法测定山奈酚血药质量浓度,色谱柱为Diamonsil C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-体积分数为0.05%磷酸溶液(体积比40∶60),流速为1.0 mL.min-1,检测波长为370 nm,柱温为40℃。结果血浆中内源性物质对山奈酚测定无干扰,线性范围为0.050～10 mg.L-1,r=0.998 5,回收率、准确度及日内、日间精密度均符合生物样品测定要求,定量下限(LLOQ)为50μg.L-1。山奈酚主要药动学参数为AUC0-t=129.2 mg.h.L-1,ρmax=7.39mg.L-1,tmax=9.0 h,t1/2=8.3 h。结论该方法简便、快速、重复性好,适用于山奈酚大鼠血药质量浓度测定及体内药动学研究。
Objective To establish an HPLC method for determining plasma level of kaempferol and investigate its pharmacokinetics in rats.Methods Rats were orally administrated with kaempferol suspension(200 mg · kg-1)and then blood samples were taken.The internal standard was phenacetin,VC was added as antioxidant.The sample was spiked with the same volume of hydrochloric acid(2 mol · L-1)and hydrolyzed for 30 min in a water bath at 80 ℃,then followed by liquid-liquid extraction.The plasma drug levels were determined by RP-HPLC,the analysis was performed on a Diamonsil C18 column(250 mm×4.6 mm,5 μm)at 40 ℃ with acetonitrile-0.05% phosphoric acid(V∶ V=40∶ 60)as the mobile phase at the flow rate of 1.0 mL · min-1.The detecting wavelength was set at 370 nm.Results The linearity was confirmed in the concentration range of 0.050-10 mg · L-1 with the correlation coefficient of 0.998 5.The limit detection(LLOQ)was 50 μg · L-1.The variability and relative recovery rate were suitable for biological analysis.The main pharmacokinetic parameters were calculated as follows:AUC0-t=129.2 mg · h · L-1,ρmax=7.39 mg · L-1,tmax=9 h,t1/2=8.3 h.Conclusions The method is convenient,rapid,accurate and can be used for pharmacokinetic study of kaempferol.

关键词(KeyWords)： 山奈酚;血药质量浓度;RP-HPLC;药动学
kaempferol;plasma concentration;RP-HPLC;pharmacokinetics

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 冀召帅,韩佳容,邹梅娟,聂鑫,王雪,程刚
JI Zhao-shuai,HAN Jia-rong,ZOU Mei-juan,NIE Xin,WANG Xue,CHENG Gang(School of Pharmacy

DOI: 10.14066/j.cnki.cn21-1349/r.2012.01.017

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