HPLC法测定雀巢速溶咖啡中胡芦巴碱的含量Determination of the content of trigonelline in the Nescafé (instant coffee) by HPLC
曲燕,赵怀清,张洪建,王学娅,李乐道,张淑华
QU Yan 1; ZHAO Huai qing 1;ZHANG Hong jian 1; WANG Xue ya 2; LI Le dao 1; ZHANG Shu hua 3 (1. School of Pharmacy;
摘要(Abstract)：
建立高效液相色谱法测定雀巢速溶咖啡中胡芦巴碱含量的方法。色谱柱为氨基键合柱 ;以乙腈 水 ( 78∶2 2 ,V∶V)为流动相 ;流速为 0 8mL/min ;检测波长为 2 6 5nm ;以外标两点法计算含量。胡芦巴碱浓度在 5 1 2～ 1 0 2 4mg/mL范围内线性关系良好 ,相关系数r=0 9999;精密度试验RSD为 0 97% (n=6 ) ;平均回收率为 98 6 % ,RSD =1 8% (n =6 )。测定 3批雀巢速溶咖啡中胡芦巴碱的含量分别为 8 4 38mg/g、8 2 5 9mg/g和 8 5 1 2mg/g,其RSD分别为 2 1 %、1 8%和 2 0 %(n =5 )。
A method was proposed for the determination of the content of trigonelline in the Nescafé (instant coffee) by HPLC. The sample was separated on Asahipak NH 2P-50 column with the mobile phase of acetonitrile water (78∶22, V∶V ). The detection was performed at 265 nm , and the flow rate was 0 8 mL/min . The result was that the calibration curve was linear in the range of 5 12～ 102 4 μg/mL . The correlation coefficient was 0 999 9 ;the RSD was 0 97%( n =6)in the precision test; The average recovery rate and RSD were 98 6% and 1 8%( n =6)respectively. The contents of trigonelline in the three batches of the Nescafé (instant coffee) and RSD were 8 438,8 259, 8 512 mg/g and 2 1%,1 8%,2 0%( n =5),respectively.
关键词(KeyWords)：
高效液相色谱法;雀巢速溶咖啡;胡芦巴碱
HPLC; Nescafé (instant coffee); trigonelline
基金项目(Foundation):
作者(Authors):
曲燕,赵怀清,张洪建,王学娅,李乐道,张淑华
QU Yan 1; ZHAO Huai qing 1;ZHANG Hong jian 1; WANG Xue ya 2; LI Le dao 1; ZHANG Shu hua 3 (1. School of Pharmacy;
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