苑振贵,陈大为,张守堂,苏书华
YUAN Zhen-gui1,CHEN Da-wei1,ZHANG Shou-tang2,SU Shu-hua3(1.School of Pharmacy

• 1:沈阳药科大学药学院
• 2:武警黑龙江省哈尔滨总队医院
• 3:沈阳军区政治部医院

摘要(Abstract)：

目的研究叶酸受体介导多西他赛长循环脂质体与肿瘤细胞的结合机理。方法采用薄膜分散法制备脂质体,采用荧光法、流式细胞仪和荧光显微镜检测脂质体与MCF-7细胞、Hela细胞的结合。结果叶酸受体介导多西他赛长循环脂质体与MCF-7细胞的结合量大于Hela细胞;游离叶酸可竞争抑制叶酸受体介导多西他赛长循环脂质体与MCF-7细胞的结合;荧光显微镜下,MCF-7细胞可见明亮绿色荧光,而Hela细胞中只有微弱绿色荧光。结论叶酸受体介导多西他赛长循环脂质体是通过叶酸介导的细胞内化而进入细胞。
Objective To investigate the tumor cells binding mechanism of the long-circulating liposomes loading docetaxel mediated by folic acid receptor(Fol-PEG-DTXL). Methods In this study,the long-circulating liposomes loading docetaxel mediated by folic acid receptor were prepared by conventional rotary-evaporated film-ultrasonication method.The flow cytometer and fluorescence microscopy were used to investigate the binding efficacy of Fol-PEG-DTXL against MCF-7 cells and Hela cells. Results After incubated with Fol-PEG-DTXL,the binding amount on MCF-7 cells was higher than that on Hela cells.The binding of Fol-PEG-DTXL could be inhabited by the addition of the free folic acid.Furthermore,a bright green fluorescence was observed on the MCF-7 cells,and the not clear green fluorescence was found on the Hela cells. Conclusions The liposomes are internalized into cells mediated by folic acid.

关键词(KeyWords)： 多西他赛;叶酸受体介导;长循环脂质体;肿瘤细胞;结合
docetaxel;folic acid receptor;long-circulating liposome;tumor cell;binding efficacy

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 苑振贵,陈大为,张守堂,苏书华
YUAN Zhen-gui1,CHEN Da-wei1,ZHANG Shou-tang2,SU Shu-hua3(1.School of Pharmacy

DOI: 10.14066/j.cnki.cn21-1349/r.2011.02.009

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