石海峰,冯宝民,史丽颖,唐玲,侯蕾,王永奇
SHI Hai-feng,FENG Bao-min,SHI Li-ying,TANG Ling,HOU Lei,WANG Yong-qi(College of Bioengineering

• 1:大连大学生物工程学院

摘要(Abstract)：

目的研究西南山茶叶的化学成分。方法采用硅胶柱色谱、Sephadex LH-20柱色谱等方法进行分离纯化,根据波谱学数据和理化性质进行结构鉴定。结果从西南山茶叶体积分数95%乙醇提取物的乙酸乙酯萃取物中分离并鉴定了11个化合物,分别为槲皮素(quercetin,1)、芦丁(rutin,2)、槲皮素-3-O-α-D-阿拉伯糖苷(quercetin-3-O-α-D-arabinofuranoside,3)、槲皮素-3-O-β-D-半乳糖苷(quercetin-3-O-β-D-galactopyranoside,4)、槲皮素-3-O-β-D-鼠李糖苷(quercetin-3-O-β-D-rhamno-side,5)、山柰酚(kaempferol,6)、山柰酚-3-O-(2″,6″-二反式-对香豆酰基)-β-D-葡萄糖苷(kaempfer-ol-3-O-[2″,6″-di-(E)-p-coumaroyl]-β-D-glucopyranoside,7)、羽扇豆醇(lupeol,8)、齐墩果酸(olean-olic acid,9)、原儿茶酸(3,4-dihydroxybenzoic acid,10)和没食子酸乙酯(ethyl gallate,11)。结论化合物1~11均首次从西南山茶中分离得到,其中化合物7为首次从山茶属植物中分离得到。
Objective To study the chemical constituents from leaves of Camellia pitardii Coh.St..Methods The compounds were isolated by chromatography on silica gel column,Sephadex LH-20 and identified on the basis of physico-chemical constants and spectral analysis.Results Eleven compounds were isolated and identified as quercetin(1),rutin(2),quercetin-3-O-α-D-arabinofurnoside(3),quercetin-3-O-β-D-galactopyranoside(4),quercetin-3-O-β-D-rhamnoside(5),kaempferol(6),kaempferol-3-O-[2″,6″-di-(E)-p-coumaroyl]-β-D-glucopyranoside(7),lupeol(8),oleanolic acid(9),3,4-dihydroxybenzoic acid(10),ethyl gallate(11).Conclusions Compounds 111 are all isolated from leaves of Camellia pitardii Coh.St.for the first time.Compound 7 is isolated from Camellia genus plants for the first time.

关键词(KeyWords)： 山茶;西南山茶;化学成分;结构鉴定
Camellia pitardii Coh.St.;Camellia;chemical constituent;structure identification

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(30772714)

作者(Author): 石海峰,冯宝民,史丽颖,唐玲,侯蕾,王永奇
SHI Hai-feng,FENG Bao-min,SHI Li-ying,TANG Ling,HOU Lei,WANG Yong-qi(College of Bioengineering

DOI: 10.14066/j.cnki.cn21-1349/r.2010.05.010

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